关键词: 有机锗, 多酸衍生物, 体外抑瘤率, 细胞周期

Abstract: BACKGROUND ＆ AIM: To study the cytotoxicity on normal cell , the inhibitive effect of Organogermanium Poly-derivatimves on cancer cell and the effect on cell cycle in vitro, and provide a basis to clinical usage. MATERIAL AND METHODS: The cytotoxicity on FL, L929 and the inhibitive rate of Organogermanium Poly-derivatimves on SMMC-7721, Hela, K562 in vitro were onducted with the method of MTT; The effect of Organogermanium Poly-derivatimves on cell cycle was studied by the way of One-Fluorescence Dying with Flow Cytometer. RESULTS: ①When the dosage reached 160.00 μg/ml, Organogermanium Poly-derivatimves did not show any cytotoxicity on FL and L929, and even stimulated cell growth; When the dosage was 160.00～1280.00 μg/ml, bigger dose showed higher cytotoxicity. ②In vitro, the inhibitive rate curve of Organogermanium Poly-derivatimves on SMMC-7721, Hela, K562 were all two-peak curve. When the dosage reached 2.50 μg/ml, the first peak of the curve, the inhibitive rate of Organogermanium Poly-derivatimves on SMMC-7721, K562 were 12.6 ％, 32.8 ％ respectively, and on Hela, When the dosage reached 5.00 μg/ml, inhibitive rate was 10.6 ％, and decreased with higher dose. When the dosage was 80.00～1 280.00 μg/ml, the inhibitive rate rised with higher dose. When the dosage reached 1 280.00 μg/ml, the inhibitive rates were 66.0 ％, 59.9 ％,65.8 ％ respectively.③When the dasage reached 5.00 μg/ml, 320.00 μg/ml, after 48 hours, Organogermanium Poly-derivatimves could extend G0/G1 and shorten S,G2 of cell cycle on SMMC-7721. CONCLUSION: At micro-dose, Organogermanium Poly-derivatimves showed obviously inhibiton to SMMC-7721,Hela,K562 and effect to cell cycle on SMMC-7221,have also observed.

Key words: organogermanium, poly-derivatimves, inhibitive rate of tumor in vitro, cell cyc