癌变·畸变·突变 ›› 2008, Vol. 20 ›› Issue (3): 216-219.doi: 10.3969/j.issn.1004-616x.2008.03.012

• 论著 • 上一篇    下一篇

杠柳苷对BT_549细胞增殖的抑制作用与p16、p27表达关系的研究

张引娟 鹿 刚 张丽杰 单保恩   

  1. 河北医科大学第四医院科研中心，石家庄　050011
  • 收稿日期:2007-09-10 修回日期:2007-12-20 出版日期:2008-05-30 发布日期:2008-05-30
  • 通讯作者: 单保恩

The Relationship between Suppressive Effects of Cortex Periplocae on BT_549 Cells and Expression of p16 and p27

ZHANG Yin-juan, LU Gang, ZHANG Li-jie, SHAN Bao-en   

  1. Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China
  • Received:2007-09-10 Revised:2007-12-20 Online:2008-05-30 Published:2008-05-30
  • Contact: SHAN Bao-en

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摘要： 背景与目的： 观察杠柳苷对人乳腺癌细胞株BT_549体外增殖抑制的作用，检测p16和p27的表达，探讨杠柳苷抑制乳腺癌细胞的可能作用机制。 材料与方法： 以不同浓度的杠柳苷(2.5、5.0、10.0 μg/ml)干预BT_549细胞，并设空白对照组,干预24、48、72 h后，分别用MTT比色法分析细胞增殖情况；流式细胞技术检测细胞凋亡与周期分布。半定量RT_PCR、流式细胞技术检测细胞p16、p27 mRNA和蛋白表达水平。 结果： 与对照组相比较，杠柳苷各实验组BT_549细胞增殖水平较对照组明显下降(P<0.01)，促进细胞凋亡率明显增加(P<0.01)，实验组细胞发生G0/G1期阻滞，细胞的p16、p27表达水平显著增高(P<0.01)。 结论： 杠柳苷具有抑制BT_549细胞增殖的作用，其机制可能与促进p16和p27表达水平的增高有关。

关键词: 杠柳苷, 乳腺癌, p16, p27

Abstract: BACKGROUND AND AIM: The suppressive effects of cortex periplocae on human breast cancer BT_549 cells and the expressions of p16 and p27 were analyzed to demonstrate the possible mechanisms. MATERIALS AND METHODS: The suppressive effects of Cortex periplocae on proliferation of BT_549 cells was analyzed with MTT method. The cell cycle and apoptosis rate of BT_549 cells treated with Cortex periplocae were examined by flow cytometry. Expressions of p16 and p27 mRNA and proteins were assessed by semiquauntitative RT_PCR and flow cytometry. RESULTS: Cortex periplocae could obviously inhibit proliferation of BT_549 cells (P<0.01). Compared to control group, after treatment with Cortex periplocae, the cell number in G0/G1 phase of BT_549 cells was increased(P<0.01), the apoptosis rate was also increased significantly(P<0.01) as well as the expression of p16 and p27(P<0.05). CONCLUSION: Cortex periplocae exerted significant inhibitory effects on BT_549 cells in vitro which was probably related to improving the expressions of p16 and p27.

Key words: cortex periplocae, breast cancer, p16, p27

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