关键词: 多氯联苯, 大鼠海马神经元细胞, bcl-2, TGF-β1

Abstract: BACKGROUND AND AIM: To study the effects of polychlorinated biphenyl(PCB) on hippocampal neuron cells in rats. MATERIALS AND METHODS: Bilateral hippocampi were isolated from neonatal Wistar rats; cellular suspensions were prepared under sterile conditions. Rats were divided into control group and three experimental groups; the dosage of PCB were low (1×10-8 mol/L ); middle(1×10-7 mol/L) and high (1×10-6 mol/L);respectively. Light microscope and immunohistochemical technology were used to examine the effects of PCB on structure and function of hippocampal neuron cells in rats. RESULTS: The structural damage of hippocampal neuron cells was related to the dose of PCB; neuron cells were most seriously affected in the high dose group. Axons and dendrites disappeared to remnants; with atrophy of cell bodies. PCB could induce bcl-2 and TGF-β1 expressions in hippocampal neuron cells; bcl-2 expression was detected in control; low dose and middle dose groups; strongest in the low dose group; while bcl-2 expression was not found in the high dose group. TGF-β1 expression was positive in control group. PCB could inhibit TGF-β1 expression in low dose group ; but the expression was up-regulated by PCB in middle dose group. CONCLUSION: PCB could affect the development of hippocampal neuron cells.

Key words: polychlorinated biphenyl(PCB), hippocampal neuron cells, bcl-2, TGF-β1