关键词: 海狗油脂肪乳, 大鼠, 胚胎发育, 受精卵, 着床

Abstract: BACKGROUND AND AIM: To observe the toxic effect of seal oil emulsion on the ability of maturity, mating, fertility and early fetal growth and development. MATERIALS AND METHODS: Wistar rats were randomly allocated into 0,250,500 and 1 000 mg/kg dose groups，and given seal oil emulsion orally for 5 weeks in male rats, 2 weeks in female rats respectively before mated, and then mated for 10 days. When the female rats were confirmed pregnant, the male rats were executed and their testicle, epididymis and sperm were examined.The seal oil emulsion treatment continued until 14th day of pregnancy and the ovary, uterus and embryo of the female rats were studied. RESULTS: The treatment groups were normal in spirit, behavior, activity and hair quality but the body weight gain was slow (average most value 50 g,P<0.05). The genitalia development, sexual function, the quantity and quality of sperm were normal. The number of live embryo (litter average 2.0) , the fetal body weight (average 0.03 g), and the mating ratio in 1 000 mg/kg group was 65％(P<0.05).There were no significant differences in the pregnancy rate, the number of corpus luteum, implantation, absorbed embryo and dead embryo, the rate of lost oosperm and fetus abnormality between the treatment and the control groups (P>0.05). CONCLUSION: Within the range of experiment doses, seal oil emulsion obviously decreased body weight gain of male rats, impaired embryonic and fetal development but did not effect the development of genitalia, mating behavior, sperm and ovum maturation and live fetus.

Key words: seal oil emulsion, rat, embryonic development, oosperm, implantation