癌变·畸变·突变 ›› 2010, Vol. 22 ›› Issue (5): 366-369.doi: 10.3969/j.issn.1004-616x.2010.05.009

• 论著 • 上一篇    下一篇

喉鳞状细胞癌中PIN1基因扩增及表达异常

李　巍1,2,李海洲2,李福才1,   

  1. 1. 中国医科大学基础医学院医学遗传学教研室,辽宁 沈阳 110001；2. 锦州市中心医院，辽宁 锦州 121000
  • 收稿日期:2010-01-18 修回日期:2010-06-06 出版日期:2010-09-30 发布日期:2010-09-30
  • 通讯作者: 李福才

PIN1 gene amplification and overexpression in human laryngeal squamous cell carcinoma

LI Wei1，2, LI Hai-zhou2, LI Fu-cai1,   

  1. 1. Department of Medical Genetics, China Medical University, Shenyang 110001, Liaoning; 2. Jinzhou Central Hospital, Jinzhou 121000, Liaoning, China
  • Received:2010-01-18 Revised:2010-06-06 Online:2010-09-30 Published:2010-09-30
  • Contact: LI Fu-cai

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摘要： 目的： 研究PIN1基因在喉癌组织中的表达情况。方法： 采用差异PCR、核型分析方法检测喉鳞癌及癌旁对照组织中PIN1基因扩增情况；采用RT－PCR检测同批组织中PIN1 mRNA表达情况；采用免疫组织化学、Western Blot方法检测PIN1蛋白表达情况。 结果： 与癌旁对照组织相比，40例喉癌组织中有9例(9/40，22.5％)存在PIN1基因扩增，27例(27/40，67.5％)PIN1 mRNA过表达，26例(26/40，65％)PIN1蛋白过表达(P<0.05)。40例中17例原代培养成功，可制备中期染色体标本，其中3例(3/17，17.65％)存在19号染色体增加。 结论： 喉鳞状细胞中PIN1基因拷贝数增加，PIN1 mRNA和蛋白质过表达，可能与喉癌发生发展有关。

关键词: 喉鳞状细胞癌, PIN1基因, 基因扩增, 过表达

Abstract: 　　【ABSTRACT】 OBJECTIVE: To investigate the amplification and overexpression of PIN1 gene in human laryngeal squamous cell carcinoma. METHODS: PIN1 gene amplification(increase of gene copy number) was detected by differential PCR and Karyotype analysis. PIN1 mRNA was evaluated by RT_PCR. Furthermore, immunohistochemistry and Western Blot were employed to detect the PIN1 protein. RESULTS: Compared with nomal control, PIN1 gene amplification rate was 22.5％(9/40), while 17.65％(3/17) was found to have an increase of chromosome 19; the rate of mRNA overexpression was 67.5％(27/40). PIN1 protein was obviously upregulated, the ratio was 65％(26/40)(P<0.05). CONCLUSION: PIN1 gene copy increased, with mRNA and protein overexpression in laryngeal carcinoma. This fact may be relevant to laryngeal cancer development.

Key words: laryngeal squamous cell carcinoma, PIN1 gene, gene amplification, overexpression

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