Sema4C在喉鳞状细胞癌组织中的表达及意义

doi:10.3969/j.issn.1004-616x.2015.03.015

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (3): 230-233.doi: 10.3969/j.issn.1004-616x.2015.03.015

Sema4C在喉鳞状细胞癌组织中的表达及意义

秦杰升¹, 洪良利², 林旭妮¹, 王挥戈¹, 邱前程²

1. 汕头大学医学院第一附属医院耳鼻喉头颈外科, 广东汕头 515041;
2. 汕头大学医学院第一附属医院病理科, 广东汕头 515041

收稿日期:2015-02-05 修回日期:2015-04-03 出版日期:2015-05-30 发布日期:2015-05-30
通讯作者: 洪良利,E-mail:hong_liangli@163.com E-mail:hong_liangli@163.com
作者简介:秦杰升,E-mail:stqinjiesheng@163.com
基金资助:
汕头市2012年医疗重点科技计划项目

Expression and significance of Sema4C in laryngeal squamous carcinoma

QIN Jiesheng¹, HONG Liangli², LIN Xuni¹, WANG Huige¹, QIU Qiancheng²

1. Depertment of Otorhinolaryngology Head and Neck Surgery, First Affiliated Hospital Of Shantou University Medical College, Shantou 515041;
2. Department of Pathology, First Affiliated Hospital Of Shantou University Medical College, Shantou 515041, Guangdong, China

Received:2015-02-05 Revised:2015-04-03 Online:2015-05-30 Published:2015-05-30

摘要/Abstract

摘要：

目的:探讨脑信号蛋白4C(Sema4C)在喉鳞状细胞癌组织中的表达及意义。方法:采用免疫组化EnVision 二步法检测82例喉鳞状细胞癌及癌旁正常喉黏膜组织中Sema4C和微小染色体维持蛋白2(MCM2)蛋白的表达水平,用D2-40标记淋巴管,计数微淋巴管密度(LMVD),分析三者的表达水平变化与临床病理指标的关系。结果:Sema4C、MCM2蛋白在喉鳞状细胞癌组织中的阳性率分别为60%(49/82)及98%(80/82),均显著高于癌旁正常喉黏膜组织(P<0.01),LMVD在癌及癌旁喉黏膜中的差别无统计学意义(P=0.077); Sema4C、MCM2的表达水平与颈部淋巴结转移有关,与年龄及肿瘤T分级无显著相关。Sema4C、MCM2 蛋白表达水平和LMVD,两两间均呈正相关关系(P<均0.05)。结论:Sema4C的异常高表达与喉鳞状细胞癌的发生发展、转移及预后有关,提示Sema4C有可能成为判断喉鳞状细胞癌生物学行为及预后指标。

关键词: 喉鳞状细胞癌, 脑信号蛋白4C, 微小染色体维持蛋白2, 免疫组化, 微淋巴管密度

Abstract:

OBJECTIVE: To investigate the expression of semaphorin 4C(Sema4C) in laryngeal squamous carcinoma and its role in tumor proliferation and lymphatic metastasis. METHODS:We investigated the expression of Sema4C and MCM2 in 82 cases of laryngeal squamous carcinoma and normal laryngeal tissues by immunohistochemical technique.Furthermore, we used D2-40 to label the lymphatic microvessel and measure the lymphatic microvessel density(LMVD) to analyze their roles in laryngeal squamous carcinoma. RESULTS:The expressions of Sema4C and MCM2 in laryngeal squamous carcinoma were significantly higher than that in the normal laryngeal tissues (P<0.01), but the difference of LVMD was not significant (P0.077). The aberrant expression rates of Sema4C, MCM2 and LVMD in laryngeal squamous carcinoma were closely related to lymph node metastasis but did not correlate with age and T stage. LMVD in laryngeal squamous carcinoma should positive correlation with the expressions of Sema4C (P<0.05) and MCM2 (P<0.01)． Moreover, there was positive correlation between Sema4C and MCM2 (P<0.05)．CONCLUSION:Sema4C may play a role in the development of laryngeal squamous carcinoma. It may be possible to follow the progression of the disease and provide prognosis by detecting the expression of Sema4c.

Key words: laryngeal squamous carcinoma, Sema4C, minichromosome maintenance 2, immunohistochemistry, lymphatic microvessel density

中图分类号:

R730.2

秦杰升, 洪良利, 林旭妮, 王挥戈, 邱前程. Sema4C在喉鳞状细胞癌组织中的表达及意义[J]. 癌变·畸变·突变, 2015, 27(3): 230-233.

QIN Jiesheng, HONG Liangli, LIN Xuni, WANG Huige, QIU Qiancheng. Expression and significance of Sema4C in laryngeal squamous carcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 230-233.

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Sema4C在喉鳞状细胞癌组织中的表达及意义

Expression and significance of Sema4C in laryngeal squamous carcinoma

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