γ射线照射诱导胸腺中调节性T细胞表型改变的研究

doi:10.3969/j.issn.1004-616x.2013.03.003

癌变·畸变·突变

γ射线照射诱导胸腺中调节性T细胞表型改变的研究

刘澜涛,刘建香,苏旭*

( 辐射防护与核应急中国疾病预防控制中心重点实验室，中国疾病预防控制中心辐射防护与核安全医学所，北京 100088 )

收稿日期:2012-11-29 修回日期:2013-04-11 出版日期:2013-05-30 发布日期:2013-05-30
通讯作者: 苏旭，E-mail：suxu@nirp.cn
作者简介:刘澜涛 (1980- )，男，山东省泰安市人，博士研究生，研究方向：辐射免疫。
基金资助:
卫生行业科研专项 (201002009)

Phenotypic changes of regulatory T cells in thymus induced by ionizing radiation

LIU Lan-tao，LIU Jian-xiang，SU Xu*

(Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China)

Received:2012-11-29 Revised:2013-04-11 Online:2013-05-30 Published:2013-05-30
Contact: SU Xu，E-mail：suxu@nirp.cn

摘要/Abstract

摘要：

目的：分析γ射线照射对小鼠胸腺淋巴细胞及调节性T细胞亚群数量和表型的影响。方法：用2 Gy γ射线全身照射小鼠，同时设未照射对照组。在照射后1、4和10 d分离胸腺中的淋巴细胞，计数后利用流式细胞仪分析CD4+ T细胞、CD4+FOXP3+CD25+ Treg细胞亚群的比例以及Treg细胞中CD39、CD103等表型因子的表达水平。结果：受照后不同细胞亚群的时间响应具有显著差异。与对照组比较，胸腺细胞和CD4+CD8- T细胞数显著减少(P<0.05)，在照射后4 d达到最低水平(P<0.05)，照射后10 d明显恢复；Treg细胞在照射后1和4 d细胞存活比例与对照组比较无显著差异，而在照射后10 d显著减少(P<0.05)；同时CD4+FOXP3+CD25+ Treg/CD4+ T比值则呈现先上升后下降的现象。照射4 d后CD39+ Treg细胞比例和CD39的平均荧光强度 (mean fluorescence indensity，MFI)均显著增加(P<0.05)，CD103+ Treg细胞比例增加但CD103的MFI却减小(P<0.05)。结论：γ射线照射后初期，Treg细胞较其他胸腺细胞具有辐射抗性，机体主要呈现免疫抑制状态，其中CD39和CD103是辐射诱导Treg细胞改变的主要表型因子，提示可能介导Treg细胞发挥抑制作用。

关键词: 电离辐射, 调节性T细胞, 表型, 胸腺

Abstract:

OBJECTIVE: To analyze the radiation-influence induced changes of the number and phenotype of the thymocytes and its regulatory subsets in mice. METHODS：Mice were exposed to 2 Gy 60Co γ-ray in a whole body manner，and thymocytes were separated and counted in 1，4 and 10 d after irradiation. The percentage of CD4+ T and reg in thymocytes and the expression of CD39，CD103 in Treg were analyzed using FACS. RESULTS：There were differences in time-response to radiation. The thymocyte and CD4SP T cell numbers were decreased significantly after whole body irradiation and reached a minimum in 4 days，but recovered clearly after 10 days. However the Treg cell decreased in a manner of non-statistical difference in 4 days，but decreased significantly in 10 days. The percentage of Treg increased first then decreased in a time-response manner. The percentage of CD39+ Treg cell and MFI of CD39 were increased after 4 days’ irradiation. The percentage of CD103+ Treg cell was also increased，however its MFI was deceased after 4 days’ irradiation. CONCLUSION：Treg cells were more resistant to radiation than other thymocytes，resulting in the immune suppression of whole body. The expressions of CD39 and CD103 were differentially regulated by radiation and may play an important role in mediating suppressive function of Treg.

Key words: ionizing radiation, regulatory T cell, phenotype, thymus

刘澜涛,刘建香,苏旭. γ射线照射诱导胸腺中调节性T细胞表型改变的研究[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2013.03.003.

LIU Lan-tao，LIU Jian-xiang，SU Xu. Phenotypic changes of regulatory T cells in thymus induced by ionizing radiation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.03.003.

参考文献

[1] Tang Q，Blueston JA. The FOXP3+ regulatory T cell：a jack of all trades，master of regulation[J]. Nat Immunol，2008，9 (3)： 239-244.

[2] Sakaguchi S. Naturally arising CD4+ regulatory T cells for immunologic self-tolerance and negative control of immune response[J]. Ann Rev Immunol，2004，22：531-562.

[3] Shalev I，Schmelzle M，Robson SC，et al. Making sense of regulatory T cell suppressive function[J]. Semin Immunol，2011， 23 (4)：282-292.

[4] Fontenot JD，Rasmussen LM，Williams JL，et al. Regulatory T cell lineage specification by the forkhead transcription factor foxp3[J]. Immunity，2005，22 (3)：329-341.

[5] Sakaguchi S，Yamaguchi T，Monura T，et al. Regulatory T cells and immune tolerance[J]. Cell，2008，133 (5)：775-787.

[6] 刘澜涛，刘建香，高刚，等. 不同剂量60Co γ 射线对小鼠淋巴细胞及其调节性T细胞亚群的影响[J]. 中华放射医学与防护杂志，2013，33 (1)：14-18.

[7] Qu Y，Jin S，Zhang A，et al. Gamma-ray resistance of regulatory CD4+CD25+FOXP3+ T cells in mice[J]. Radiat Res，2010，173 (2)：148-157.

[8] Hou B，Xu ZW，Yang CW，et al. Protective effects of inosine on mice subjected to lethal total-body ionizing radiation[J]. J Radiat Res，2007，48 (1)：57-62.

[9] Hofer M，Pospisil M，Znojil V，et al. Drugs elevating extracellular adenosine promote regeneration of haematopoietic progenitor cells in severely myelosuppressed mice：their comparison and joint effects with the granulocyte colony-stimulating factor[J]. Eur J Haematol，2002，68 (1)：4-11.

[10] Cepek KL，Shaw SK，Parker CM，et al. Adhesion between epithelial cells and T lymphocytes mediated by E-cadherin and the alpha E beta7 integrin[J]. Nature，1994，372 (6502)：190-193.

[11] Chen X，Subleski JJ，Kopf H，et al. Cutting edge：expression of TNFR2 defines a maximally suppressive subset of mouse CD4+CD25+FOXP3+ T regulatory cells：applicability to tumor-infiltrating T regulatory cells[J]. J Immunol，2008，180 (10)： 6467-6471.

[1]	杨玉, 黄雅理, 林飞, 汤龙. 抗肿瘤血清胸腺因子9肽的急性毒性和遗传毒性[J]. 癌变·畸变·突变, 2022, 34(1): 57-61.
[2]	向嘉琪, 孙佳丽, 王成芳, 刘青杰, 田梅. ⁶⁰Co γ射线全身照射大鼠尿液的非标记定量蛋白质组学分析[J]. 癌变·畸变·突变, 2021, 33(3): 172-177,192.
[3]	陆雪, 田雪蕾, 赵骅, 蔡恬静, 田梅, 刘青杰. 人外周血淋巴细胞微核分析用于估算离体模拟局部照射剂量的研究[J]. 癌变·畸变·突变, 2021, 33(3): 193-196.
[4]	丁雅雯, 焦文静, 王芃堉, 鹿刚, 马鸣. 多发性骨髓瘤患者浆细胞免疫表型特点及其临床意义研究[J]. 癌变·畸变·突变, 2020, 32(6): 464-468,489.
[5]	赵骅, 陆雪, 蔡恬静, 李爽, 田梅, 刘青杰. 基于代谢组学方法筛选大鼠血浆辐射敏感代谢物[J]. 癌变·畸变·突变, 2020, 32(3): 166-170,176.
[6]	李爽, 陆雪, 封江彬, 田梅, 蔡恬静, 刘青杰. 性别和年龄对⁶⁰Co γ射线照射离体人外周血辐射敏感基因mRNA表达的影响[J]. 癌变·畸变·突变, 2019, 31(6): 421-427.
[7]	田雪蕾, 封江彬, 田梅, 刘青杰. 甲基化芯片检测电离辐射诱导人外周血淋巴细胞DNA甲基化水平的变化[J]. 癌变·畸变·突变, 2019, 31(6): 434-439.
[8]	齐雪松, 王春燕, 李辰, 佟鹏, 邵帅, 曲功霖, 苟巧, 苏旭. γ射线对小鼠胸腺淋巴细胞微丝形态及丝切蛋白磷酸化的影响[J]. 癌变·畸变·突变, 2018, 30(4): 249-253,257.
[9]	高延晓, 田梅, 高刚, 刘建香. 降扎乡高氡温泉周围居民血浆中8-羟基脱氧鸟苷和硫氧还蛋白还原酶水平的研究[J]. 癌变·畸变·突变, 2018, 30(3): 226-229.
[10]	黎青叶, 柳晓玲, 吴小嫩, 郭萍, 陈雯, 陈丽萍. 组蛋白H3(Ser10)磷酸化在维持肿瘤细胞恶性表型中的作用[J]. 癌变·畸变·突变, 2018, 30(2): 81-86,91.
[11]	曲功霖, 李辰, 邵帅, 王春燕, 齐雪松, 佟鹏, 李宁. 四君子汤对电离辐射损伤的防治作用[J]. 癌变·畸变·突变, 2017, 29(4): 295-299.
[12]	张忠新, 张睿凤, 张慧芳, 段志凯. 常用辐射生物剂量计的研究现状[J]. 癌变·畸变·突变, 2016, 28(4): 325-328.
[13]	杨英杰, 刘建香, 高刚. 电离辐射对小鼠外周血单核细胞亚群的影响[J]. 癌变·畸变·突变, 2016, 28(4): 298-301.
[14]	许文黎, 岳娟, 袁慧, 黄立群, 王新钢, 王永丽, 闻建华. 氮氧自由基在辐射防护中的应用[J]. 癌变·畸变·突变, 2015, 27(6): 490-492.
[15]	董小梅, 王治, 张国伟, 董建云, 周妮娅, 凌曦, 刘晋祎, 曹佳, 张丽龙, 敖琳. 低剂量工业射线探伤工人职业暴露的遗传损伤效应[J]. 癌变·畸变·突变, 2015, 27(2): 101-105.

γ射线照射诱导胸腺中调节性T细胞表型改变的研究

Phenotypic changes of regulatory T cells in thymus induced by ionizing radiation

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价