Toll样受体基因多态性与EBV感染相关胃癌的易感性

doi:10.3969/j.issn.1004-616x.2014.03.003

癌变·畸变·突变

Toll样受体基因多态性与EBV感染相关胃癌的易感性

赵真真，石源源，刘颂，王笑峰，罗兵*

山东省青岛大学医学院微生物学教研室，山东青岛 266021

收稿日期:2014-01-10 修回日期:2014-04-09 出版日期:2014-05-30 发布日期:2014-05-30
通讯作者: 罗兵，E-mail：qdluobing@163.com
作者简介:赵真真(1987- )，女，山东青岛市人，硕士研究生，研究方向：EBV相关肿瘤。E-mail： qdzzz1987@ gmail.com
基金资助:
国家自然科学基金(30970157)，山东省自然科学基金(ZR2011CM016)

Toll-like receptors gene polymorphism and susceptibility to EBV- associated gastric carcinoma

ZHAO Zhen-zhen，SHI Yuan-yuan，LIU Song，WANG Xiao-feng，LUO Bing*

Department of Medical Microbiology, Qingdao University Medical College, Qingdao 266021, Shandong, China

Received:2014-01-10 Revised:2014-04-09 Online:2014-05-30 Published:2014-05-30

摘要/Abstract

摘要：

目的：探讨Toll样受体(TLR)家族成员TLR2基因启动子区-196~-174 del和TLR4基因 Thr399Ile位点多态性与EBV相关胃癌(EBVaGC)及EBV阴性胃癌(EBVnGC)易感性的关系。方法：采用PCR技术检测52例EBVaGC，157例EBVnGC以及94例正常对照人群TLR2(-196~-174 del)基因多态性；PCR-限制性片段长度多态性(PCR-RELP)技术检测50例EBVaGC，67例EBVnGC以及71例正常对照TLR4 Thr399Ile位点基因型及等位基因分布；分析2种基因多态性与EBVaGC及EBVnGC易感性的关系。结果：胃癌组与对照组比较TLR2(-196~-174 del)基因型分布无显著差异(χ2=3.180，P=0.075)，胃癌组del等位基因频率明显高于对照组(χ2=4.875， P=0.027)，del等位基因携带者的罹患危险性明显高于非携带者(OR=1.491，95%CI=1.045~2.126)；EBVaGC组和EBVnGC组中，TLR2(-196~-174 del)3种基因型以及del等位基因频率差异无统计学意义(χ2=0.05，P=0.867)。EBVaGC组、EBVnGC组和正常对照组中均未发现TLR4基因Thr399Ile位点的多态，其基因型分布及等位基因频率差异均无统计学意义(P>0.05)。结论：TLR2(-196~-174 del)等位基因可能是胃癌发病危险因素，且在EBVaGC和EBVnGC两种胃癌发生中产生相同的影响。未发现TLR2(-196~-174 del)和TLR4基因Thr399Ile基因型分布与EBVaGC的易感性相关。

关键词: Toll样受体, 胃癌, EB病毒, 基因多态性

Abstract:

OBJECTIVE: To explore the relationship of Toll-like receptors 2 (TLR2) and TLR4 Thr399Ile gene polymorphism and the susceptibility to EBV-associated gastric carcinoma (EBVaGC) and EBV-negative gastric carcinoma (EBVnGC). METHODS：TLR2 gene promoter region - 196 to - 174 del polymorphism was detected by using the allele-specific polymerase chain reaction in 52 EBVaGCs，157 EBVnGCs and 94 normal controls. TLR4 gene Thr399Ile genotype and allele distribution were evaluated by PCR and restriction fragment length polymorphism (PCR-RFLP) in 50 EBVaGCs，67 EBVnGCs and 71 normal controls. The relationship between gene polymorphism in TLR2 and TLR4 coding genes and the susceptibility to EBVaGC and EBVnGC was analysed. RESULTS：There was no significant difference (χ2=3.180，P=0.075) between gastric cancer group and control group in genotype distribution of TLR2(-196 to -174 del)，but the del allele frequency of gastric cancer group was obviously higher than that of control group (χ2=4.875，P=0.027)，The risk of developing gastric carcinoma in del allele carriers was significantly higher than that in controls(OR=1.491，95%CI= 1.045-2.126). TLR2 (-196 to -174 del) genotype and the frequency of del allele showed no significant difference in EBVaGC and EBVnGC group (χ2=0.05，P=0.867). TLR4 gene Thr399Ile locus mutation was not found in EBVaGC，EBVnGC or normal control groups. The genotype distribution and allele frequency also revealed no statistical differences . CONCLUSION：TLR2 -196 to - 174 del alleles could be risk factors for gastric carcinoma，but its effects were not different in EBVaGC and EBVnGC. There was no obvious correlation in the genotype distribution of TLR2(-196 to -174 del) and TLR4 gene Thr399Ile and EBVaGC genetic susceptibility.

Key words: Toll-like receptor, gastric carcinoma, EBV, gene polymorphism

赵真真，石源源，刘颂，王笑峰，罗兵*. Toll样受体基因多态性与EBV感染相关胃癌的易感性[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2014.03.003.

ZHAO Zhen-zhen，SHI Yuan-yuan，LIU Song，WANG Xiao-feng，LUO Bing*. Toll-like receptors gene polymorphism and susceptibility to EBV- associated gastric carcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.03.003.

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Toll样受体基因多态性与EBV感染相关胃癌的易感性

Toll-like receptors gene polymorphism and susceptibility to EBV- associated gastric carcinoma

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