白细胞介素-17过表达胃癌细胞在小鼠体内的成瘤效应研究

doi:10.3969/j.issn.1004-616x.2019.04.006

癌变·畸变·突变 ›› 2019, Vol. 31 ›› Issue (4): 295-299.doi: 10.3969/j.issn.1004-616x.2019.04.006

白细胞介素-17过表达胃癌细胞在小鼠体内的成瘤效应研究

张智萍^1,3, 单保恩¹, 宋静静¹, 王浩¹, 刘英姿¹, 陈伟¹, 高立平¹, 李巧霞²

1. 河北医科大学第四医院科研中心, 河北石家庄 050011;
2. 河北医科大学第四医院临床生物细胞室, 河北石家庄 050011;
3. 河北医科大学第二医院特检科, 河北石家庄 050011

收稿日期:2018-09-20 修回日期:2019-01-08 出版日期:2019-07-30 发布日期:2019-08-23
通讯作者: 李巧霞,E-mail:759767368@qq.com E-mail:759767368@qq.com
作者简介:张智萍,E-mail:550539269@qq.com。
基金资助:
河北省自然科学基金（H2016206209）；河北省人才工程培养经费项目（A201401251）；河北省政府资助临床医学优秀人才项目

Tumorigenic effect of interleukin?17 overexpressing gastric cells in mice

ZHANG Zhiping^1,3, SHAN Baoen¹, SONG Jingjing¹, WANG Hao¹, LIU Yingzi¹, CHEN Wei¹, GAO Liping¹, LI Qiaoxia²

1. Research Centre of the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011;
2. The Clinical Biological Cell Room of the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011;
3. Exceptional Laboratory Department of the Second Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China

Received:2018-09-20 Revised:2019-01-08 Online:2019-07-30 Published:2019-08-23

摘要/Abstract

摘要： 目的：建立小鼠荷瘤模型，研究白细胞介素-17（IL-17）过表达的胃癌细胞在小鼠体内的成瘤效应。方法：分别以MFC、MFC/pcDNA3.1和MFC/IL-17细胞接种615小鼠，细胞浓度为5×10⁶个/mL，按每只200 μL注射于小鼠背部皮下。每组10只，观察小鼠皮下肿瘤生长情况。3周后处死小鼠，分别采用RT-PCR和Western blot法检测肿瘤组织内IL-17、VEGF、Podoplanin mRNA和蛋白表达；免疫组化法检测微血管密度。结果：MFC/IL-17组小鼠于接种后5~7 d开始形成肿瘤结节，且生长迅速，肿瘤质量为（5.384±0.391）g，大于MFC组[（1.726±0.445）g]和MFC/pcDNA3.1组[（2.064±0.397）g]（P < 0.05）。RT-PCR和Western blot结果显示，与MFC和MFC/pcDNA3.1组相比，MFC/IL-17组小鼠肿瘤组织内IL-17、VEGF、Podoplanin mRNA和蛋白表达均明显增加（P < 0.05）。免疫组化结果显示，与MFC和MFC/pcDNA3.1组相比，MFC/IL-17组小鼠肿瘤组织内微血管密度明显增加（P < 0.05）。结论：IL-17可能通过上调VEGF、Podoplanin和CD31的表达促进血管和淋巴管形成，加速肿瘤生长。

关键词: IL-17, 胃癌细胞, 血管形成, 促肿瘤作用

Abstract: OBJECTIVE:The aim was to investigate tumorigenic effects of interleukin-17 (IL-17) overexpressing gastric cancer cells in mice. METHODS:MFC,MFC/pcDNA3.1 and MFC/IL-17 cells were inoculated subcutaneously into 615 mice,with 10 mice in each group. Three weeks after the inoculation when tumors became obvious,mRNA and protein expressions of IL-17,vascular endothelial growth factor (VEGF) and Podoplanin in tumor tissues were detected using RT-PCR and Western-blots. Micro-vessel density was detected by immunohistochemistry. RESULTS:Tumor nodules developed on 5-7 d after inoculation and grew rapidly in mice inoculated with MFC/IL-17 (MFC/IL-17 mice). The average tumor weights in MFC/IL-17 mice[(5.384±0.391) g] were significantly more than that in MFC mice[(1.726±0.445) g] and MFC/pcDNA3.1 mice[(2.064±0.397) g] (P < 0.05). In addition,the mRNA and protein expressions of IL-17,VEGF and Podoplanin in MFC/IL-17 mice were significantly higher than that in MFC and MFC/pcDNA3.1 mice (P < 0.05). The micro-vessel densities were significantly increased in MFC/IL-17 mice than that in MFC and MFC/pcDNA3.1 mice (P < 0.05). CONCLUSION:Our data show that expression of IL-17 accelerated tumor growth in vivo by up-regulating the expression of VEGF,Podoplanin and CD31,thus promoting angiogenesis and lymphatic vessel formation.

Key words: IL-17, gastric cancer cells, angiogenesis, tumor-promoting effect

中图分类号:

R735.2

张智萍, 单保恩, 宋静静, 王浩, 刘英姿, 陈伟, 高立平, 李巧霞. 白细胞介素-17过表达胃癌细胞在小鼠体内的成瘤效应研究[J]. 癌变·畸变·突变, 2019, 31(4): 295-299.

ZHANG Zhiping, SHAN Baoen, SONG Jingjing, WANG Hao, LIU Yingzi, CHEN Wei, GAO Liping, LI Qiaoxia. Tumorigenic effect of interleukin?17 overexpressing gastric cells in mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(4): 295-299.

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白细胞介素-17过表达胃癌细胞在小鼠体内的成瘤效应研究

Tumorigenic effect of interleukin?17 overexpressing gastric cells in mice

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