整合素αvβ6和JunB在口腔鳞癌组织中的表达及其临床意义

doi:10.3969/j.issn.1004-616x.2015.03.016

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (3): 234-238,241.doi: 10.3969/j.issn.1004-616x.2015.03.016

整合素αvβ6和JunB在口腔鳞癌组织中的表达及其临床意义

方少斌^1,3, 邓小玲², 陈筠¹, 陈永辉⁴, 许铭炎^1,2

1. 厦门市口腔医院, 福建厦门 361003;
2. 厦门大学医学院基础医学部, 福建厦门 361102;
3. 汕头大学医学院第二附属医院口腔科, 广东汕头 515041;
4. 福建医科大学附属漳州医院口腔科, 福建漳州 363000

收稿日期:2015-03-23 修回日期:2015-04-22 出版日期:2015-05-30 发布日期:2015-05-30
通讯作者: 陈永辉,E-mail:CYH1501@163.com;许铭炎,E-mail:mingyan_xu@qq.com E-mail:CYH1501@163.com;mingyan_xu@qq.com
作者简介:方少斌,E-mail:377970040@qq.com;邓小玲,E-mail:xiaolingdeng@xmu.edu.cn
基金资助:
国家自然科学基金项目(30900661,81072208,81370160);中央高校基本业务费专项资金项目(厦门大学);福建省医学创新科研项目(2014-CXB-47);福建省自然科学基金青年项目

The expression and clinical relevance of integrin αvβ6 and JunB in oral squamous cell carcinoma

FANG Shaobin^1,3, DENG Xiaoling², CHEN Jun¹, CHEN Yonghui⁴, XU Mingyan^1,2

1. Xiamen Stomatology Hospital, Xiamen 361003, Fujian;
2. Department of Basic Medical Science, Xiamen Universtiy Medical College, Xiamen 361102, Fujian;
3. Department of Stomatology, The Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong;
4. Department of Stomatology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000, Fujian, China

Received:2015-03-23 Revised:2015-04-22 Online:2015-05-30 Published:2015-05-30

摘要/Abstract

摘要：

目的:探讨整合素αvβ6、JunB在口腔鳞癌组织中的表达及其临床意义。方法:采用免疫组化方法检测120例口腔鳞癌及15例口腔正常黏膜组织中整合素αvβ6和JunB的表达情况,并分析两者表达与口腔鳞癌患者临床病理指标及预后的关系。结果:整合素αvβ6表达于口腔鳞癌组织的细胞质和胞膜,而JunB定位在口腔鳞癌组织和口腔正常黏膜组织的细胞核内。整合素αvβ6、JunB在口腔鳞癌组织中的表达强度显著高于口腔正常黏膜组织(P<0.01)。整合素αvβ6在口腔鳞癌组织中的表达与浸润深度(T分期)、组织分化程度、是否有淋巴结转移(N分期)密切相关(P<0.05),而JunB在口腔鳞癌组织中的表达与浸润深度、是否有淋巴结转移密切相关(P<0.05)。单因素生存分析表明,整合素αvβ6高表达患者的预后较低表达的患者差(P=0.021)。Cox回归多因素分析表明是否有淋巴结转移是影响患者预后的独立危险因素(P<0.05)。结论:整合素αvβ6和JunB在口腔鳞癌组织中表达增加,并与口腔鳞癌的浸润、转移密切相关;整合素αVβ6表达可作为判断口腔鳞癌患者预后判断的指标。

关键词: 整合素αvβ6, 口腔鳞癌, JunB, 免疫组织化学

Abstract:

OBJECTIVE: This study aimed to investigate the expression of integrin αvβ6 and JunB in oral squamous cell carcinoma (OSCC) and the correlation with clinicopathologic characteristics and prognosis. METHODS:The expression of integrin αvβ6 and JunB protein was detected with immunohistochemical staining assay in 120 cases of OSCC and 15 samples of normal oral mucosa. The correlation with clinicopathologic characteristics and prognosis of the patients were evaluated. RESULTS:Integrin αvβ6 was detected in both cytoplasm and cellular membrane of OSCC, but JunB protein was localized in the nuclei of both OSCC and normal oral mucous tissues. The expressions of integrin αvβ6 and JunB protein in OSCC were significantly higher than that in normal oral mucosa (P<0.01). The expression of integrin αvβ6 was positively related to depth of invasion(T stage), tumor differentiation degree and lymph node metastasis(N stage) (P<0.05). The expression of JunB was positively related to depth of invasion and lymph node metastasis (P<0.05). Survival as a single factor analysis revealed that patients with over-expression of integrin αvβ6 had a shorter overall survival(OC) (P=0.021). Through Cox multivariate regression analysis, lymph node metastasis (N stage) was an independent risk factor that affected the prognosis. CONCLUSION: The over-expressions of integrin αvβ6 and JunB were common in OSCC and closely correlated with tumor invasion and metastasis. Integrin αvβ6 may be used as a prognostic marker for therapy.

Key words: integrin αvβ6, oral squamous cell carcinoma, JunB, immunohistochemistry

中图分类号:

R394.3

方少斌, 邓小玲, 陈筠, 陈永辉, 许铭炎. 整合素αvβ6和JunB在口腔鳞癌组织中的表达及其临床意义[J]. 癌变·畸变·突变, 2015, 27(3): 234-238,241.

FANG Shaobin, DENG Xiaoling, CHEN Jun, CHEN Yonghui, XU Mingyan. The expression and clinical relevance of integrin αvβ6 and JunB in oral squamous cell carcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 234-238,241.

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整合素αvβ6和JunB在口腔鳞癌组织中的表达及其临床意义

The expression and clinical relevance of integrin αvβ6 and JunB in oral squamous cell carcinoma

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