三氯乙烯药疹样皮炎患者血清miRNAs差异表达谱分析

doi:10.3969/j.issn.1004-616x.2016.06.011

癌变·畸变·突变 ›› 2016, Vol. 28 ›› Issue (6): 468-471.doi: 10.3969/j.issn.1004-616x.2016.06.011

三氯乙烯药疹样皮炎患者血清miRNAs差异表达谱分析

郑剑^1,2, 蓝涛², 刘威², 邓荣霞², 李培茂³, 张艳芳³, 刘建军^1,2

1. 湖南师范大学医学院预防医学系, 湖南长沙 410013;
2. 深圳市疾病预防控制中心, 广东深圳 518055;
3. 深圳市职业病防治院, 广东深圳 518001

收稿日期:2016-07-12 修回日期:2016-11-01 出版日期:2016-11-30 发布日期:2016-11-30
通讯作者: 刘建军,E-mail:junii8@126.com E-mail:junii8@126.com
作者简介:郑剑,E-mail:653242262@qq.cm。
基金资助:
国家自然科学基金（81273126）；深圳市基础研究计划项目（JCYJ20150402102135497）；深圳市学科布局项目（JCYJ20160428142316603）

Differentially expressed miRNA profile in workers with trichloroethylene-induced medicamentosa-like dermatitis

ZHENG Jian^1,2, LAN Tao², LIU Wei², DENG Rongxia², LI Peimao³, ZHANG Yanfang³, LIU Jianjun^1,2

1. Department of Preventive Medicine, Medicine School of Hunan Normal University, Changsha 410013, Hunan;
2. Shenzhen Center for Disease Control & Prevention, Shenzhen 518055, Guangdong;
3. Shenzhen Prevention and Treatment Center for Occupational Disease, Shenzhen 518001, Guangdong, China

Received:2016-07-12 Revised:2016-11-01 Online:2016-11-30 Published:2016-11-30

摘要/Abstract

摘要： 目的：分析三氯乙烯药疹样皮炎（OMLDT）患者和三氯乙烯接触者血清microRNA（miRNA）表达谱的差异。方法：分别采集5名三氯乙烯药疹样皮炎患者和5名接触者血液，分离血清，抽提总RNA进行Hy3荧光标记，然后浓缩标记的样品，运用miRCURY LNA芯片进行miRNA芯片杂交，杂交后进行图像扫描，所得数据使用GenePix Pro V6.0软件进行miRNA差异表达分析并采用qPCR进行验证。对差异较大的miRNA用miRWalk 2.0进行靶基因预测，并与其他预测软件结果进行比较。结果：OMLDT患者与三氯乙烯接触者比较，共有69个差异表达的miRNAs，其中低表达30个，高表达39个（包含miR-199b）。qPCR验证亦显示miR-199b在OMLDT中高表达。靶基因预测结果显示miR409-3p和miR485-3p在5个预测软件均预测到了共同的靶基因。结论：三氯乙烯药疹样皮炎患者血清miRNAs表达谱发生了显著改变，miR-199b可能是OMLDT潜在的易感生物标志物。

关键词: miRNA, 三氯乙烯药疹样皮炎, 靶基因预测, 生物标志物

Abstract: OBJECTIVE: To profile microRNAs expression among workers with trichloroethylene (TCE)-induced medicamentosa-like dermatitis (OMLDT). METHODS: Serum samples from 5 patients with OMLDT and with TCE contacts were collected. The total RNA was extracted then labeled with Hy3 fluorescent. MicroRNAs were hybridized with miRCURY LNA chips and images were acquired using the GenePix 4000B scanner,qPCR method was used to verify the results of miRNA microarray. The results were further analyzed by prediction softwares. RESULTS: A total of 69 microRNAs were differentially expressed between OMLDT and TCE contacts. Among the microRNAs,39 were up-regulated and 30 were down-regulated. Increase of miR-199b-5p was verified using qPCR. Target prediction results showed that the same target genes of miR-409-3p and miR-485-3p had been predicted in all forecasting software. CONCLUSION: The study showed that expression of microRNA in serum of workers with OMLDT was significantly altered,and miR-199b might serve as a candidate biomarker for OMLDT.

Key words: miRNA, occupational medicamentosa-like dermatitis induced by trichloroethylene, target gene prediction, biomarkers

中图分类号:

R394.6

郑剑, 蓝涛, 刘威, 邓荣霞, 李培茂, 张艳芳, 刘建军. 三氯乙烯药疹样皮炎患者血清miRNAs差异表达谱分析[J]. 癌变·畸变·突变, 2016, 28(6): 468-471.

ZHENG Jian, LAN Tao, LIU Wei, DENG Rongxia, LI Peimao, ZHANG Yanfang, LIU Jianjun. Differentially expressed miRNA profile in workers with trichloroethylene-induced medicamentosa-like dermatitis[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(6): 468-471.

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三氯乙烯药疹样皮炎患者血清miRNAs差异表达谱分析

Differentially expressed miRNA profile in workers with trichloroethylene-induced medicamentosa-like dermatitis

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