某氡温泉周边居民外周血淋巴细胞中miRNAs的表达

doi:10.3969/j.issn.1004-616x.2015.06.008

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (6): 446-449.doi: 10.3969/j.issn.1004-616x.2015.06.008

某氡温泉周边居民外周血淋巴细胞中miRNAs的表达

刘春旭, 李小亮, 潘艳, 阮健磊, 高刚, 田梅, 刘建香

中国疾病预防控制中心辐射防护与核安全医学所, 辐射防护与核应急中国疾病预防控制中心重点实验室, 北京 100088

收稿日期:2015-06-15 修回日期:2015-11-02 出版日期:2015-11-30 发布日期:2015-11-30
通讯作者: 刘建香,E-mail:jxliu@163.com E-mail:jxliu@163.com
作者简介:刘春旭,E-mail:liuchunxu0214@126.com。
基金资助:
国家自然科学基金项目(31100606)

Expression of miRNAs in peripheral blood lymphocytes of the residents around hot springs with radon

LIU Chunxu, LI Xiaoliang, PAN Yan, RUAN Jianlei, GAO Gang, TIAN Mei, LIU Jianxiang

Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China

Received:2015-06-15 Revised:2015-11-02 Online:2015-11-30 Published:2015-11-30

摘要/Abstract

摘要： 目的:探讨河北省某地氡温泉对周围居民外周血淋巴细胞中miR-16、miR-106b、miR-449a、miR-34a和let-7g表达的影响。方法:采用简单随机抽样法抽取某地氡温泉周边居民43人;同时随机抽取生活习惯相似,但未长期接触过氡温泉的居民44人。采用实时荧光定量PCR(qPCR)方法检测研究对象淋巴细胞中miRNAs的表达水平。用t检验比较miRNA在氡温泉组和对照组居民外周血淋巴细胞中的差异表达;采用多重线性回归分析miRNA的差异性表达和居民氡暴露的关系。结果:与对照组相比,氡温泉组居民外周血淋巴细胞中miR-106b、miR-449a和let-7g表达水平显著上调(t值分别为4.180、2.422、2.794,P均<0.05)。控制年龄、性别、吸烟和饮酒等因素后,miR-106b、miR-449a和let-7g的差异性表达和居民氡暴露因素呈正相关(t值分别为4.091、2.716、2.440,P均<0.05)。但是miR-16和miR-34a的表达改变与氡暴露无显著相关(t值分别为0.990、0.147,P均>0.05)。结论:miR-106b、miR-449a和let-7g具有作为氡致辐射损伤早期标志物的潜在价值。

关键词: 氡温泉, 淋巴细胞, miRNAs, 辐射损伤

Abstract: OBJECTIVE: To investigate the expressions of miR-16,miR-106b,miR-449a,miR-34a and let-7g in peripheral blood lymphocytes of the residents around hot springs with radon in Hebei province. METHODS: Two groups were randomly selected in Hebei province,Radon group consisted of 43 residents around hot springs with radon and control group were 44 residents with similar living conditions but not around hot springs. qRT-PCR was used to detect miRNAs level in radon and control groups. t test was used for statistical analysis of miRNA levels in lymphocytes between two groups. Multiple linear regression analyse were employed to test the association between miRNAs expression and radon exposure. RESULTS: Compared with control group,the levels of miR-106b,miR-449a and let-7g in lymphocytes of radon group were significantly increased(t=4.180,2.422,2.794;P<0.05). After controlling of confounding factors such as age,gender,smoking and drinking,alteration of miR-106b,miR-449a and let-7g in lymphocytes were positively correlated with radon exposure(t=4.091,2.716,2.440; P<0.05). No significant difference was observed in the lymphocyte levels of miR-16 and miR-34a between the two groups(t=0.990,0.147;P>0.05). CONCLUSION: MiR-106b,miR-449a and let-7g levels could potentially used as biomarkers for radon exposure.

Key words: hot springs with radon, lymphocyte, miRNAs, radiation injury

中图分类号:

Q345+.1

刘春旭, 李小亮, 潘艳, 阮健磊, 高刚, 田梅, 刘建香. 某氡温泉周边居民外周血淋巴细胞中miRNAs的表达[J]. 癌变·畸变·突变, 2015, 27(6): 446-449.

LIU Chunxu, LI Xiaoliang, PAN Yan, RUAN Jianlei, GAO Gang, TIAN Mei, LIU Jianxiang. Expression of miRNAs in peripheral blood lymphocytes of the residents around hot springs with radon[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 446-449.

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某氡温泉周边居民外周血淋巴细胞中miRNAs的表达

Expression of miRNAs in peripheral blood lymphocytes of the residents around hot springs with radon

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