维吾尔族女性乳腺癌与低分子量蛋白酶体蛋白表达缺失的关系

doi:10.3969/j.issn.1004-616x.2017.02.015

癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (2): 146-150.doi: 10.3969/j.issn.1004-616x.2017.02.015

维吾尔族女性乳腺癌与低分子量蛋白酶体蛋白表达缺失的关系

古丽扎热叶·艾库拉¹, 木克代斯·拜克提亚尔¹, 贺春钰¹, 艾秀清¹, 张玉晶², 木妮热·木沙江¹

1. 新疆医科大学附属肿瘤医院乳腺放疗科, 新疆乌鲁木齐 830011;
2. 中山大学肿瘤防治中心放疗科, 广东广州 510060

收稿日期:2017-01-16 修回日期:2017-02-23 出版日期:2017-03-31 发布日期:2017-03-31
通讯作者: 木妮热·木沙江,E-mail:muni66@aliyun.com E-mail:muni66@aliyun.com
作者简介:古丽扎热叶·艾库拉,E-mail:gulzarya@163.com。
基金资助:
新疆维吾尔自治区科技支疆项目（201491178）

Loss of expression of low molecular-weight proteins in breast cancers of Uighur women

GULZARIA·Aikula¹, MUKADDAS·Bahtiyar¹, HE Chunyu¹, AI Xiuqing¹, ZHANG Yujing², MUNIRE·Musa¹

1. Department of Breast Radiotherapy, Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang;
2. Department of Radiation Oncology, Sun Yat-sen University Cancer Center, Guangzhou 510060, Guangdong, China

Received:2017-01-16 Revised:2017-02-23 Online:2017-03-31 Published:2017-03-31

摘要/Abstract

摘要：

目的：探讨低分子量蛋白酶体（LMP）蛋白表达与乳腺癌的关系及其在维吾尔族和汉族女性中的表达差异。方法：收集维吾尔族和汉族女性乳腺纤维腺瘤及乳腺癌患者的石蜡包埋组织标本165例，应用免疫组织化学SP法检测LMP2、LMP7、LMP10蛋白的表达水平。结果：与乳腺纤维腺瘤组相比，LMP2和LMP7蛋白在乳腺癌组织中表达水平显著下降，表达完全缺失率明显上升，分别为67%和46.1%，差异均具有统计学意义（P < 0.01）；LMP10在乳腺纤维腺瘤和乳腺癌组中的表达缺失率分别为48%和56.5%，两组之间表达差异无统计学意义（P > 0.05）。LMP2、LMP7和LMP10蛋白在维吾尔族女性乳腺癌患者中的表达率分别为43.1%、43.1%和47.7%，汉族分别为46%、42%和42%，不同民族女性患者之间表达的差异均无统计学意义（P均>0.05）。LMP2、LMP7和LMP10与乳腺癌淋巴结转移及远处转移相关，差异均有统计学意义（P均<0.05），同时LMP10表达降低与肿块大小有明显关系（P < 0.01）。结论：LMP蛋白表达水平降低或缺失可能与乳腺癌的发生有关，其变化趋势在维吾尔族和汉族间有共性，可能反映乳腺癌发生的分子机制和特有的遗传背景。

关键词: 低分子量蛋白酶体, 乳腺癌, 维吾尔族女性

Abstract:

OBJECTIVE: To compare the expression of low molecular-weight proteins (LMP) in breast cancers between Uighur and Han women. METHODS: We collected 165 cases of paraffin-embedded breast fibro adenoma or breast cancer tissues from Uighur and Han women and analyzed the protein expression of LMP2,LMP7 and LMP10 by immunohistochemistry. RESULTS: The total loss rate of LMP2 and LMP7 was significantly increased in breast cancer (67%/46.1%),the normal expression level was reduced with a significant difference (P < 0.01),the alternation trend of the loss rate of LMP10 was common to breast cancer and breast fibro adenoma (breast fibro adenoma 48%,breast cancer 56.5%),and the difference between the two groups is not statistically significant (P > 0.05). The alteration trend of the expression of LMP2,LMP7 and LMP10 was common to women from both Uighur and Han ethnic groups,in Uighur groups (43.1%,43.1%,47.7%),in Han groups (46%,42%,42%) and the difference between two ethnic groups is not statistically significant (P > 0.05). However,LMP2,LMP7 or LMP10 were significantly correlated with tumor lymph node invasion and distant metastasis (P < 0.05),LMP10 was significantly correlated with tumor size (P < 0.01). CONCLUSION: The loss of expression of LMP protein may be an important marker for breast cancer and was common to women from both Uighur and Han ethnic groups. This suggest that the loss may be a molecular mechanism for breast cancer and for both ethnic background.

Key words: low molecular-weight protein, breast cancer, Uighur women

中图分类号:

R737.9

古丽扎热叶·艾库拉, 木克代斯·拜克提亚尔, 贺春钰, 艾秀清, 张玉晶, 木妮热·木沙江. 维吾尔族女性乳腺癌与低分子量蛋白酶体蛋白表达缺失的关系[J]. 癌变·畸变·突变, 2017, 29(2): 146-150.

GULZARIA·Aikula, MUKADDAS·Bahtiyar, HE Chunyu, AI Xiuqing, ZHANG Yujing, MUNIRE·Musa. Loss of expression of low molecular-weight proteins in breast cancers of Uighur women[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(2): 146-150.

参考文献

[1] BODAI BI,TUSO P. Breast cancer survivorship:a comprehensive review of long-term medical issues and lifestyle recommendations[J]. Perman J,2015,19(2):48-79.

[2] BLUM JS,WEARSCH PA,CRESSWELL P. Pathways of antigen processing[J]. Annu Rev Immunol,2013,31:443-473.

[3] MIZUKAMI Y,KONO K,MARUYAMA T,et al. Downregulation of HLA class I molecules in the tumour is associated with a poor prognosis in patients with oesophageal squamous cell carcinoma[J]. British J Cancer,2008,99(9):1462-467.

[4] MEHTA A M,JORDANOVA E S,KENTER G G,et al. Association of antigen processing machinery and HLA class I defects with clinicopathological outcome in cervical carcinoma [J]. Cancer Immun Immunother,2008,57(2):197-206.

[5] SO T,TAKENOYAMA M,MIZUKAMI M,et al. Haplotype loss of HLA class I antigen as an escape mechanism from immune attack in lung cancer[J]. Cancer Res,2005,65(13):5945-5952.

[6] BANDOH N,OGINO T,KATAYAMA A,et al. HLA class I antigen and transporter associated with antigen processing downregulation in metastatic lesions of head and neck squamous cell carcinoma as a marker of poor prognosis[J]. Oncol Rep,2010,23(4):933-939.

[7] AYSHAMGUL H,MA H,ILYAR S,et al. Association of defective HLA-I expression with antigen processing machinery and their association with clinicopathological characteristics in Kazak patients with esophageal cancer[J]. Chin Med J,2011,124(3):341-346.

[8] TANAKA K. The proteasome:overview of structure and functions[J]. Proceed Jpn Acad,2009,85(1):12-36.

[9] TOWNSEND A R,ROTHBARD J,GOTCH F M,et al. The epitopes of influenza nucleoprotein recognized by cytotoxic T lymphocytes can be defined with short synthetic peptides[J]. J Immunol,1986,44(6):959-968.

[10] LUESCHER I F. A nonapeptide encoded by human gene MAGE-1 is recognized on HLAA1 by cytolytic T lymphocytes directed against tumor antigen MZ2-E by catia traversari,Pierre van der Bruggen[J]. J Exp Med,1992,176(5):1453-1457.

[11] OHTA Y,FLAJNIK M F. Coevolution of MHC genes (LMP/TAP/class Ia,NKT-class Ib,NKp30-B7H6):lessons from cold-blooded vertebrates[J]. Immunol Rev,2015,267(1):6-15.

[12] ZHENG Y,PARSONAGE G,ZHUANG X,et al. Human leukocyte antigen (HLA) A*1101-restricted Epstein-Barr virus-specific T-cell receptor gene transfer to target nasopharyngeal carcinoma[J]. Cancer Immunol Res,2015,3(10):1138-1147.

[13] KHAN A N H,GREGORIE C J,TOMASI T B. Histone deacetylase inhibitors induce TAP,LMP,Tapasin genes and MHC class I antigen presentation by melanoma cells[J]. Cancer Immuno Immunother,2008,57(5):647-654.

[14] WEHENKEL M,BAN J O,HO Y K. Selective inhibitor of the immunoproteasome subunit LMP2 induces apoptosis in PC-3 cells and suppresses tumour growth in nude mice[J]. Br J Cancer,2012,107(1):53-62.

[15] QIAO L,CHUN YAN H,PENG S,et al. Down-regulation of HLA class I antigen-processing machinery components in esophageal squamous cell carcinomas:Association with disease progression[J]. Gastroenterology,2009,44:960-969.

[16] FAVOLE A,CASCIO P,CERRUTI F,et al. MHC class I-related antigen-processing machinery component defects in feline mammary carcinoma[J]. Transl Oncol,2012,5(1):48-55.

[17] 成芳,张茜,刘卫华,等. 新疆2019例汉族、维吾尔族乳腺癌临床病理资料分析[J]. 肿瘤防治研究,2010,37(11):1312-1314.

[18] 木妮热·木沙江,籍素芝,古丽扎热叶·艾库拉. 新疆地区乳腺癌危险因素调查研究[J]. 临床肿瘤学杂志,2016,21 (1):34-40.

[19] 籍素芝,古丽扎热叶·艾库拉,朱相露,等. 新疆维吾尔族和汉族乳腺癌与HLA-DRB1基因多态性的分析[J]. 中华肿瘤防治杂志,2015,22(23):1791-1796.

[20] 木妮热·木沙江,古丽扎热叶·艾库拉,贺春钰,等. 新疆维吾尔族女性乳腺癌与HLA-DRB1基因多态性的关系及临床意义[J]. 癌变·畸变·突变,2015,27(5):366-369.

[21] GOBBI G,MIRANDOLA P,MICHELONI C. Expression of HLA class I antigen and proteasome subunits LMP-2 and LMP-10 in primary vs. metastatic breast carcinoma lesions[J]. Int J Oncol,2004,25(6):1625-1629.

[22] BENDER C M,ZINGG J M,JONES P A,et al. DNA methylation as a target for drug design[J]. Pharm Res,1998,15(2):175-187.

[23] AYXAMGUL H,GULZARYA A,ABULIZI A,et al. Post-transcriptional and epigenetic regulation of antigen processing machinery (APM) components and HLA-I in cervical cancers from Uighur women[J]. PLoS One,2012,7(9):44952.

[24] 古丽扎热叶·艾库拉,阿仙姑·哈斯木,阿布力孜·阿布杜拉,等. 维吾尔族妇女宫颈病变及人乳头状瘤病毒感染与抗原处理相关转运体TAP1和TAP2基因启动子甲基化的关系[J]. 中华病理学杂志,2012,47(7):438-442.

[25] 阿仙姑·哈斯木,哈提拉·吐尔逊,阿布力孜·阿布杜拉,等. 维吾尔族妇女宫颈病变组织中HPV感染与低相对分子质量蛋白酶体2和7基因启动子甲基化的关系[J]. 中华微生物学和免疫学杂志,2012,32(3):205-211.

[26] 阿仙姑·哈斯木. 维吾尔族宫颈癌发生与抗原呈递元件基因表达调控的关系研究[D]. 乌鲁木齐:新疆医科大学,2012.

[27] ASHLEY G R,WENDELL D J, RISHER J D,et al. DNA methylation-dependent epigenetic regulation of gene expression in MCF7 breast cancer cells[J]. Epigenetics,2006,1(1):32-44.

维吾尔族女性乳腺癌与低分子量蛋白酶体蛋白表达缺失的关系

Loss of expression of low molecular-weight proteins in breast cancers of Uighur women

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	辛雨濛, 梁桓熙, 余悦华, 孙震晓. 基于网络药理学和分子对接法预测甘草干姜汤抗乳腺癌的主要活性成分及作用机制[J]. 癌变·畸变·突变, 2022, 34(1): 7-13.
[2]	赵岩, 孙震晓. 二甲双胍抑制人MDA-MB-231细胞增殖及迁移的体外实验研究[J]. 癌变·畸变·突变, 2022, 34(1): 35-39.
[3]	方煜彤, 张群琛, 洪超群, 陈春发, 陈炯玉, 张任栋, 吴俊东. 乳腺癌中miR-221、miR-222表达水平对GATA3和FOXA1蛋白的影响及其作用机制[J]. 癌变·畸变·突变, 2021, 33(6): 435-441,460.
[4]	张世民, 赵瑞君. 蛋白酪氨酸磷酸酶受体J在乳腺癌组织中的表达及其与临床预后的相关性[J]. 癌变·畸变·突变, 2021, 33(4): 276-279,285.
[5]	程斌, 王新宇, 孙钰, 张家泰, 董淑英. 二甲双胍对DEHP和DBP诱导的MCF-7细胞增殖和迁移的抑制作用[J]. 癌变·畸变·突变, 2021, 33(3): 197-202.
[6]	罗娇, 张坤驰, 周莉. 三阴性乳腺癌细胞系MDA-MB-231与其脑转移细胞系中microRNA差异表达分析[J]. 癌变·畸变·突变, 2021, 33(3): 218-224.
[7]	李玮玮, 朱莹. 数据挖掘分析MYB家族在乳腺癌中的表达及其预后评估价值[J]. 癌变·畸变·突变, 2020, 32(6): 414-422.
[8]	陈绵龄, 马硕一. miR-21通过靶向PDCD4调控三阴性乳腺癌细胞的迁移和侵袭[J]. 癌变·畸变·突变, 2020, 32(4): 292-297.
[9]	张惠娟, 黄大元, 张洁, 唐海欧, 谢安心, 谭敦勇. SF1a-PRLR和ΔS2 SF1a-PRLR对乳腺癌MCF-7细胞增殖和microRNA表达的影响[J]. 癌变·畸变·突变, 2020, 32(3): 203-208.
[10]	孙全武, 杜小平, 郝杰, 时荣, 刘波, 黄海云. 伴病理性乳头溢液的乳腺癌患者癌组织整合素β1的表达及意义[J]. 癌变·畸变·突变, 2019, 31(5): 368-372.
[11]	王兰, 张永东, 郭红云, 王涛, 王海涛, 郭欢, 杨碎胜, 李晓琴, 苏海翔. XRCC3基因多态性与西北地区妇女乳腺癌的关联性研究[J]. 癌变·畸变·突变, 2019, 31(5): 392-396,400.
[12]	梁辰, 靳翠红. 乳腺癌易感基因1调控氧化损伤在神经退行性疾病中作用的研究进展[J]. 癌变·畸变·突变, 2019, 31(3): 257-260.
[13]	杜娟, 胡向阳, 刘丹, 胡卫, 陈涛. 透骨草总生物碱对乳腺癌骨转移的抑制作用及其机制[J]. 癌变·畸变·突变, 2018, 30(6): 435-441.
[14]	肖红梅, 潘天齐, 董淑楠, 付凌萌, 王少康, 孙桂菊. 绝经状态影响饮茶与乳腺癌患病风险关系的meta分析[J]. 癌变·畸变·突变, 2018, 30(5): 395-399.
[15]	姜朋涛, 胡志芳, 高兴春, 郭娜, 张典, 姜凤良. p53在调控DNA损伤所致MDAMB-231细胞死亡中的作用及其机制[J]. 癌变·畸变·突变, 2018, 30(5): 349-353.