抗肿瘤新药乙烷硒啉对人舌鳞状细胞癌细胞增殖和迁移的影响

doi:10.3969/j.issn.1004-616x.2018.04.007

癌变·畸变·突变 ›› 2018, Vol. 30 ›› Issue (4): 279-285.doi: 10.3969/j.issn.1004-616x.2018.04.007

抗肿瘤新药乙烷硒啉对人舌鳞状细胞癌细胞增殖和迁移的影响

邢龙^1,2, 李杨^3,4, 马小龙^3,4, MOHAMMED H^3,4, 黄莹^3,4, 谢富强⁴, 冯正虎^1,2

1. 西北民族大学口腔医学国家民委重点实验室, 甘肃兰州 730030;
2. 西北民族大学甘肃省口腔疾病研究重点实验室/培育基地, 甘肃兰州 730030;
3. 兰州大学口腔医学院, 甘肃兰州 730000;
4. 兰州大学第二医院, 甘肃兰州 730030

收稿日期:2018-03-15 修回日期:2018-06-26 出版日期:2018-07-30 发布日期:2018-07-30
通讯作者: 谢富强,E-mail:xiefq@lzu.edu.cn E-mail:xiefq@lzu.edu.cn
作者简介:邢龙,E-mail:499768964@qq.com
基金资助:
西北民族大学引进人才科研项目（XbMuyjrc201319）

Effects of ethaselen, a novel organoselenium compound, on proliferation and migration of human tongue squamous carcinoma cells

XING Long^1,2, LI Yang^3,4, MA Xiaolong^3,4, MOHAMMED H^3,4, HUANG Ying^3,4, XIE Fuqiang⁴, FENG Zhenghu^1,2

1. Key Lab of Stomatology of State Ethnic Affairs Commission, Northwest Minzu University, Lanzhou 730030;
2. Key Lab of Oral Diseases of Gansu Province, Northwest Minzu University, Lanzhou 730030;
3. School of Stomatology Lanzhou University, Lanzhou 730000;
4. The Second Hospital of Lanzhou University, Lanzhou 730030, Gansu, China

Received:2018-03-15 Revised:2018-06-26 Online:2018-07-30 Published:2018-07-30

摘要/Abstract

摘要： 目的：探讨新型有机硒化合物乙烷硒啉（BBSKE）对人舌癌CAL27细胞株生长和增殖的影响。方法：分别采用体外细胞实验MTT、Hoechst 33258免疫荧光染色、Annexin V-FITC/PI染色流式细胞术、Transwell小室实验检测2、5、10、20 μmol/L（含1‰ DMSO）的BBSKE对CAL27细胞增殖、细胞周期及迁移的影响，并设置溶剂对照组和正常对照组。结果：5、10、20 μmol/L的BBSKE作用CAL27细胞后，其增殖抑制率较对照组明显增加（P < 0.01），且随作用时间的延长而增加。Hoechst 33258免疫荧光染色结果显示5、10 μmol/L BBSKE组相比对照组细胞出现凋亡形态改变。Annexin V-FITC/PI染色流式细胞术分析结果显示与对照组相比，2、5、10、20 μmol/L BBSKE组CAL27细胞G2/M期比率呈梯度增加（P < 0.01），细胞周期被阻滞于G2/M期（P < 0.01）。Transwell小室实验结果显示，CAL27细胞的迁移数量在2、5、10、20 μmol/L BBSKE组相比对照组明显减少（P < 0.01），且迁移细胞数与药物浓度呈反比。结论：BBSKE对CAL27细胞具有增殖抑制作用，其机制可能是通过引起细胞周期阻滞于G2/M期而诱导细胞凋亡，并且抑制细胞迁移，提示BBSKE可作为舌鳞状细胞癌患者化疗治疗的备选药物。

关键词: 乙烷硒啉, 硫氧还蛋白, CAL27细胞, 细胞增殖, 细胞周期, 凋亡, 迁移

Abstract: OBJECTIVE: To investigate the effect of a new type of organic selenium compound ethaselen (BBSKE) on growth and proliferation of a human tongue cancer CAL27 cell line. METHODS: In vitro cell assay MTT,Hoechst 33258 immunofluorescence staining,annexin V-FITC/PI staining flow cytometry,transwell chamber assay were performed to determine effects of 2,5,10,20 μmol/L (containing 1‰ DMSO) of BBSKE on CAL27 proliferation,and cell cycle and migration compared with solvent control and normal control groups. RESULTS: CAL27 cells treated with 5,10,and 20 μmol/L BBSKE had significantly reduced proliferation compared with untreated cultures (P < 0.01),and with further reduction in time. With the Hoechst 33258 immunofluorescence staining,the morphology of cells in the 5 and 10 μmol/L BBSKE groups was changed compared to the control group. Annexin V-FITC/PI staining flow cytometry results showed that the G2/M phase ratios in the 2,5,10,and 20 μmol/L BBSKE group were increased compared to the control group (P < 0.01),with blockage in the G2/M phase (P < 0.01). Results from the transwell chamber assay showed that the number of CAL27 cells exhibiting migration in the 2,5,10,and 20 μmol/L BBSKE groups were significantly lower than that in the control group (P < 0.01). The number of migrating cells in the 20 μmol/L BBSKE group was the least. In addition,the migration number and the concentration of drug were inversely proportional. CONCLUSION: BBSKE demonstrated significant inhibitory effect on CAL27 cells,induced cell apoptosis by arresting the cell cycle in the G2/M phase and inhibited cell migration. These observations suggest that BBSKE can be used as an alternative drug for chemotherapy in patients with TSCC.

Key words: ethaselen, thioredoxin, CAL27 cells, cell proliferation, cell cycle, apoptosis, cell migration

中图分类号:

邢龙, 李杨, 马小龙, MOHAMMED H, 黄莹, 谢富强, 冯正虎. 抗肿瘤新药乙烷硒啉对人舌鳞状细胞癌细胞增殖和迁移的影响[J]. 癌变·畸变·突变, 2018, 30(4): 279-285.

XING Long, LI Yang, MA Xiaolong, MOHAMMED H, HUANG Ying, XIE Fuqiang, FENG Zhenghu. Effects of ethaselen, a novel organoselenium compound, on proliferation and migration of human tongue squamous carcinoma cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(4): 279-285.

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抗肿瘤新药乙烷硒啉对人舌鳞状细胞癌细胞增殖和迁移的影响

Effects of ethaselen, a novel organoselenium compound, on proliferation and migration of human tongue squamous carcinoma cells

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