X连锁RNA结合蛋白对食管癌Eca109细胞生物学行为的影响

doi:10.3969/j.issn.1004-616x.2025.05.010

癌变·畸变·突变 ›› 2025, Vol. 37 ›› Issue (5): 405-410.doi: 10.3969/j.issn.1004-616x.2025.05.010

• 论著 • 上一篇

X连锁RNA结合蛋白对食管癌Eca109细胞生物学行为的影响

海妮萨依姆·图尔荪¹, 张志强², 刘玲¹, 张琪琪², 李卉¹

1. 新疆医科大学基础医学院生物化学与分子生物学教研室, 新疆乌鲁木齐 830054;
2. 新疆医科大学第一附属医院消化病二科, 新疆乌鲁木齐 830054

收稿日期:2024-08-03 修回日期:2025-02-27 发布日期:2025-10-17
通讯作者: 李卉
作者简介:海妮萨依姆·图尔荪，E-mail：2624932797@qq.com。
基金资助:
新疆维吾尔自治区科技创新团队(天山创新团队)项目(2022TSYCTD0018)

Effects of RNA-binding motif protein X-linked on biological behavior of esophageal carcinoma cells

HAINISAYIM·Tursun¹, ZHANG Zhiqiang², LIU Ling¹, ZHANG Qiqi², LI Hui¹

1. Department of Biochemistry and Molecular Biology, School of Basic Medicine, Xinjiang Medical University, Urumqi 830054;
2. The Second Department of Gastroenterology, the First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang, China

Received:2024-08-03 Revised:2025-02-27 Published:2025-10-17

摘要/Abstract

摘要： 目的：探讨X连锁RNA结合蛋白(RBMX)对食管癌Eca109细胞生物学行为的影响，进一步阐明其可能的作用机制。 方法：将对数生长期的 Eca109 细胞分为对照组和实验组，利用 Lipofectamine^TM 3000 转染 RBMX 特异性小干扰 RNA(siRNA)及过表达载体，分别用逆转录实时荧光定量PCR(RT-qPCR)和Western blot法检测各细胞组中RBMX的mRNA和蛋白表达水平。采用CCK-8法、Transwell小室实验、Western blot法分别检测转染后细胞增殖能力、迁移能力及EMT标志分子蛋白表达的变化。 结果：与对照组比较，转染RBMX siRNA后，Eca109细胞中RBMX的mRNA和蛋白表达水平明显降低(P<0.05)；转染过表达载体后，细胞中RBMX 的 mRNA 和蛋白表达水平明显升高(P<0.05)。siRNA 转染 Eca109 细胞 24 h 后，细胞的增殖、迁移能力明显减弱(P<0.05)，E-cadherin的蛋白表达水平升高而N-cadherin和vimentin的蛋白表达水平降低(P<0.05)；转染过表达载体48 h后，Eca109细胞的增殖、迁移能力明显增强(P<0.05)，E-cadherin 的蛋白表达水平下降但 N-cadherin 和 vimentin 的蛋白表达水平升高(P<0.05)。 结论：RBMX能影响Eca109细胞的增殖和迁移能力，并可能通过影响EMT过程参与食管癌的发展。

关键词: 食管鳞癌, X连锁RNA结合蛋白, 小干扰RNA, 上皮间质转化

Abstract: OBJECTIVE：To investigate mechanisms of RNA-binding motif protein X-linked(RBMX) on biological behavior of esophageal cancer cells in vitro. METHODS：Logarithmic Eca109 cells in culture were divided into control and experimental groups. Lipofectamine^TM 3000 was used to transfect small interfering RNA (siRNA) and overexpression vector of RBMX. The mRNA and protein expression levels of RBMX were detected by RT-qPCR and Western blot，respectively. CCK-8，Transwell and Western blot were used to detect changes of cell proliferation， migration and EMT marker protein expression after transfection. RESULTS： Following transfection with RBMX siRNA， the mRNA and protein expression levels of RBMX in Eca109 cells were markedly reduced compared to the control group (P<0.05). Conversely， transfection with the overexpression vector resulted in a significant increase in RBMX mRNA and protein expression levels (P<0.05). After 24 hours of siRNA transfection，proliferation and migration of Eca109 cells were significantly inhibited (P<0.05)， accompanied by an increase in E-cadherin protein expression and a decrease in N-cadherin and Vimentin protein expression (P<0.05). In contrast，48 hours post-transfection with the overexpression vector，proliferative and migratory capabilities of Eca109 cells were notably enhanced (P<0.05)，with decreased E-cadherin protein expression and increased N-cadherin and Vimentin protein expression (P<0.05). CONCLUSION： RBMX influenced the proliferation and migration of Eca109 cells in vitro，potentially contributing to esophageal cancer development through its impact on the epithelial-mesenchymal transition process.

Key words: esophageal squamous cell carcinoma, RNA binding motif protein X-linked, siRNA, epithelialmesenchymal transition

中图分类号:

R735.1

海妮萨依姆·图尔荪, 张志强, 刘玲, 张琪琪, 李卉. X连锁RNA结合蛋白对食管癌Eca109细胞生物学行为的影响[J]. 癌变·畸变·突变, 2025, 37(5): 405-410.

HAINISAYIM·Tursun, ZHANG Zhiqiang, LIU Ling, ZHANG Qiqi, LI Hui. Effects of RNA-binding motif protein X-linked on biological behavior of esophageal carcinoma cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2025, 37(5): 405-410.

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X连锁RNA结合蛋白对食管癌Eca109细胞生物学行为的影响

Effects of RNA-binding motif protein X-linked on biological behavior of esophageal carcinoma cells

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