关键词: 大蒜素, 人食管癌, EC9706细胞, 诱导作用

Abstract:

OBJECTIVE: To study the apoptotic effect of garlicin on esophageal cancer EC9706 cells. METHODS: We used 18 μg/mL garlicin to treat esophageal cancer EC9706 cells，and analyzed the effect by cell culture，cell count，flow cytometry analysis，Hoechst33258 staining，HE staining and electron microscopy. RESULTS：Proliferation of EC9706 cells could be inhibited by 18 μg/mL garlic，cell growth inhibition rate was 75.89%. Flow cytometry analysis showed that garlic could induce the emergence of apoptosis，and the rate was 23.8%，blocked at G2/M phase. Concentrated cell nucleus appeared granular and fluorescent by Hoechst33258 staining. Light microscope and electron microscope showed that the morphology of the cells treated with 18 μg/mL garlic appeared shrunken，cell nucleus concentrated，chromatin agglutination，mitochondria swelling，and other significant apoptosis characteristics. CONCLUSION：This study suggested that garlic could induce apoptosis of esophageal cancer EC9706 cells effectively.

Key words: garlicin, human esophageal cancer, EC9706 cells, induction role