Hh信号通路在口腔鳞状细胞癌中的激活及意义

doi:10.3969/j.issn.1004-616x.2013.02.006

癌变·畸变·突变

Hh信号通路在口腔鳞状细胞癌中的激活及意义

王俊菊,宋令岗,张凯,刘艳平

滕州市中心人民医院，山东枣庄 277500

收稿日期:2012-08-30 修回日期:2012-12-29 出版日期:2013-03-30 发布日期:2013-03-30
通讯作者: 王俊菊,-mail：wjjslg@163.com.cn
作者简介:王俊菊 (1984– ），女，山东省枣庄市人，硕士，研究方向：E-mail：wjjslg@163.com.cn

The activation and function of Hh signaling pathway in oral squamous cell carcinoma

WANG Jun-ju，SONG Ling-gang，ZHANG Kai，LIU Yan-ping

Tengzhou Central People’s Hospital, Zaozhuang 277500, Shandong, China

Received:2012-08-30 Revised:2012-12-29 Online:2013-03-30 Published:2013-03-30
Contact: WANG Jun-ju,-mail：wjjslg@163.com.cn

摘要/Abstract

摘要：

目的：研究口腔鳞状细胞癌中Shh、Ptch、Gli-1在基因及蛋白水平的表达，并探讨其阳性表达与口腔鳞状细胞癌生物学特性之间的关系。方法：采用免疫组织化学方法分别检测40例口腔鳞癌和30例正常口腔黏膜中Shh、Ptch、Gli-1蛋白的表达；RT-PCR法检测10例口腔鳞癌组织及5例正常口腔黏膜组织中Shh、Gli-1和Ptch mRNA的表达。结果：免疫组织化学结果显示，Shh、Ptch和Gli-1蛋白在口腔鳞癌中阳性表达率分别为62.5%、60.0％和65.0％，在正常口腔黏膜中3种蛋白均不表达。非参数统计分析示，Shh及Gli-1的阳性表达与肿瘤的大小、淋巴结转移及临床分期相关 (P<0.05)，Ptch蛋白的表达与淋巴结转移呈显著正相关 (P<0.05)。RT-PCR结果显示，Shh、Ptch、Gli-1 mRNA在口腔鳞癌组织中的阳性表达分别为60%、50%、70%，明显高于正常口腔黏膜，差异有统计学意义 (P<0.05)。结论：Hh信号通路在口腔鳞状细胞癌组织中被激活并与口腔鳞癌的发生、发展、转移关系密切。

关键词: 口腔鳞状细胞癌, Sonic hedgehog, Patched, Gli-1

Abstract:

OBJECTIVE: To examine the expressions of Shh，Gli1 and Ptch，the key elements of Hedgehog signaling pathway in oral squamous cell carcinoma and normal oral mucosa，and to explore the relationship between their expressions and biological parameters. METHODS：The expressions of Shh，Ptch，Gli1 were examined in 40 cases of oral squamous cell carcinoma and 30 normal oral mucosa (as control) by immunohistochemistry. The expressions of Shh，Gli1，Ptch mRNA were analyzed in specimens from 10 oral squamous cell carcinoma and 5 nomal oral specimens by RT-PCR . RESULTS：There was no expression of Shh，Ptch，Gli1 in 30 specimens of normal oral mucosa. The positive expression rates of Shh，Ptch，Gli-1 in oral squamous cell carcinoma were 62.5%，60.0%，65.0%，respectively. Nonparametric Mann-Whitney test showed that the expressions of Shh and Gli-1 were related to the tumor size，lymph node metastasis and clinical stages (P<0.05). But the expression of Ptch was related to metastasis (P<0.05). A statistically significant correlation was found among the expressions of Shh，Ptch and Gli-1 (rs=0.527，0.406，0.578，respectively， P<0.01). Shh，Ptch and Gli-1 mRNA in 60%，50%，70%，respectively，of oral squamous cell carcinoma were higher than in nomal oral mucosa，the difference was statistically significant (P<0.05). CONCLUSION：The activation of hedgehog signaling pathway in oral squamous cell carcinoma and hedgehog signaling pathway may be related to the development and metastasis of oral squamous cell carcinoma.

Key words: oral squamous cell carcinoma, Sonic hedgehog, Patched, Gli-1

王俊菊,宋令岗,张凯,刘艳平. Hh信号通路在口腔鳞状细胞癌中的激活及意义[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2013.02.006.

WANG Jun-ju，SONG Ling-gang，ZHANG Kai，LIU Yan-ping. The activation and function of Hh signaling pathway in oral squamous cell carcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.02.006.

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Hh信号通路在口腔鳞状细胞癌中的激活及意义

The activation and function of Hh signaling pathway in oral squamous cell carcinoma

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