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表没食子儿茶素没食子酸酯对人成淋巴细胞株hMLH1 和hMSH2 mRNA表达的影响

 娟1,李 珍1,薛京伦2,汪 旭1,*   

  1. 1. 云南师范大学生命科学学院,生物能源持续开发与利用教育部工程研究中心,云南 昆明 650500;2. 复旦大学遗传学研究所,上海 200433
  • 收稿日期:2013-02-21 修回日期:2013-04-29 出版日期:2013-07-30 发布日期:2013-07-30
  • 通讯作者: 汪 旭,E-mail:wangxu@fudan.edu.cn
  • 作者简介:倪 娟 (1982- ),女,在读博士研究生,实验师,研究方向:分子营养基因组。E-mail:gt_gg30@yahoo.com.cn.
  • 基金资助:

    国家自然科学基金 (30960166,31260268);联合基因生物医药有限公司合作项目;云南省科技项目 (2010ZC065)

Effects of (-)epigallocatechin gallate on expressions of hMLH1 and hMSH2

NI Juan1LI Zhen1,XUE Jing-lun2,WANG Xu1,*   

  1. 1. School of Life Sciences, Yunnan Normal University, Engineering Research Center of Sustainable Development and Utilizaton of Biomass Energy, Ministry of Education, Kunming 650500, Yunnan; 2. Institute of Genetics, Fudan University, Shanghai 200433, China
  • Received:2013-02-21 Revised:2013-04-29 Online:2013-07-30 Published:2013-07-30
  • Contact: WANG Xu,E-mail:wangxu@fudan.edu.cn

摘要:

目的: 探讨表没食子儿茶素没食子酸酯[(-)epigallocatechin gallate ,EGCG]对人成淋巴细胞株错配修复基因hMLH1和hMSH2 mRNA表达水平的影响。方法:将正常人成淋巴细胞株GM12593和乳腺癌患者成淋巴细胞株GM13705分别置于含有0、5、10、20 μmol/L EGCG的RPMI-1640中进行6 d干预培养后,采用实时荧光定量PCR (FQ-PCR)技术检测干预前后错配修复基因hMLH1和hMSH2 mRNA表达水平的变化。结果:经20 μmol/L EGCG干预培养6 d后,GM12593细胞hMLH1与hMSH2 mRNA表达水平均显著高于其他浓度组 (P均<0.05),且显著高于同等浓度时GM13705细胞中上述2个基因的表达水平(P<0.05);EGCG对GM13705 目标基因的表达无显著影响 (P>0.05)。结论:EGCG具有上调正常人成淋巴细胞株hMLH1与hMSH2 mRNA表达水平的潜力,可能通过增加错配修复起始复合物的数量,来帮助错配修复机制的启动,维护基因组的稳定性。

关键词: 表没食子儿茶素没食子酸酯, 人成淋巴细胞株, 错配修复基因, 基因表达

Abstract:

OBJECTIVE: To investigate the effect of (-) epigallocatechin gallate(EGCG) on mRNA expressions of hMLH1 and hMSH2 in human lymphoblast cell lines. METHODS: Total RNA was isolated from GM12593 and GM13705 cells treated with EGCG(0,5,10,20 μmol/L) for 6 days. Real-time fluorecence quantification PCR(FQ-PCR) was conducted to measure the mRNA expression levels of hMLH1 and hMSH2. RESULTS: The mRNA expressions of hMLH1 and hMSH2 significantly increased at 20 μmol/L EGCG in GM12593 cells,and were significantly higher than equal concentrations in GM13705 cells. The effect of EGCG on expressions of target genes were insignificant in GM13705 cells. CONCLUSION: EGCG had the potential to up-regulate the expressions of hMLH1 and hMSH2, contributed to the promotion of mismatch repair and maintaining genomic stability in normal human lymphoblast cell line GM12593.

Key words: (-)epigallocatechin gallate, human lymphoblast cell line, mismatch repair gene, gene expression