甲醛对HepG2细胞脂肪代谢的影响

doi:10.3969/j.issn.1004-616x.2013.06.003

癌变·畸变·突变

甲醛对HepG2细胞脂肪代谢的影响

闫丹丹,白剑英*,梁瑞峰,王幼萍,雷佩玉

( 山西医科大学公共卫生学院环境卫生学教研室，山西太原 030001 )

收稿日期:2013-09-03 修回日期:2013-10-07 出版日期:2013-11-30 发布日期:2013-11-30
通讯作者: 白剑英，E-mail：jybai66@aliyun.com
作者简介:闫丹丹 (1986- )，女，陕西省渭南市人，硕士研究生，研究方向：环境污染物的肝脏毒性。
基金资助:
山西省回国留学人员科研资助项目 (2009-53)

Effects of formaldehyde on lipid metabolism in HepG2 cells

YAN Dan-dan，BAI Jian-ying*，LIANG Rui-feng，WANG You-ping，LEI Pei-yu

(Department of Environmental Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, Shanxi, China)

Received:2013-09-03 Revised:2013-10-07 Online:2013-11-30 Published:2013-11-30
Contact: BAI Jian-ying，E-mail：jybai66@aliyun.com

摘要/Abstract

摘要：

目的： 探讨甲醛对HepG2细胞甘油三酯 (triglyceride，TG)含量的影响及其可能机制。方法：以0.02、0.1、0.5、2.5、12.5 mmol/L的甲醛 (formaldehyde，FA)分别处理人肝癌HepG2细胞24和48 h，采用CCK-8法检测细胞活性，GPO-POD 酶法测定细胞内TG含量。另分别以0.004、0.02、0.1 mmol/L的甲醛处理人肝癌HepG2细胞24和48 h，采用Western blot法检测肉碱棕榈酰转移酶1 (carnitine palmitoyl transferase 1，CPT1)、固醇调节元件结合蛋白-1c (sterol regulatory element-binding protern-1c，SREBP-1c)、腺苷酸核糖基化因子1 (ADP-ribosylation factor 1，Arf1)和包被蛋白Ⅰ (coat protein comlex Ⅰ，COPⅠ)的蛋白表达水平。结果：与对照组比较，0.5～12.5 mmol/L FA染毒24 h或0.1～12.5 mmol/L染毒48 h 时可显著降低HepG2细胞活性 (P均<0.05)；0.1 mmol/L FA染毒24 h，或者0.1 和0.02 mmol/L FA染毒48 h，均可致HepG2细胞内TG含量显著升高 (P均<0.05)；FA染毒48 h引起肝细胞内CPT1表达水平明显降低 (P均<0.05)，SREBP-1c和COPⅠ表达水平在染毒24和48 h后均增高 (P均<0.05)，FA染毒24 h后 Arf1表达水平明显增加 (P<0.05)。结论：FA接触可引起HepG2细胞内TG含量增加，其机制可能与脂肪酸β氧化受到抑制和脂肪合成增加有关。

关键词: 甲醛, 甘油三酯, 肉碱棕榈酰转移酶1, 固醇调节元件结合蛋白-1c, 腺苷酸核糖基化因子1, 包被蛋白Ⅰ

Abstract:

OBJECTIVE: To investigate the effects and possible mechanism of liquid formaldehyde on the triglyceride content of HepG2 hepatocytes. METHODS：HepG2 cells were treated with formaldehyde in 0.02，0.1，0.5，2.5 and 12.5 mmol/L concentrations，CCK-8 method was used to detect cell viability. Triglyceride was measured by triglyceride GPO-POD assay kit after formaldehyde exposure. The expression of carnitine palmitoyl transferase 1 (CPT1)，sterol regulatory element-binding protein (SREBP) 1c，ADP-ribosylation factor 1 (Arf1) and coat protein comlex Ⅰ(COPⅠ) were assessed by western blot after treatment with formaldehyde in 0.004，0.02，0.1 mmol/L concentrations. RESULTS：0.5－12.5 mmol/L FA exposure of 24 h or 0.1-12.5 mmol/L FA exposure of 48 h could significantly reduce the viability of HepG2 cells (P<0.05). TG contents were increased significantly after exposure to 0.1 mmol/L FA for 24 hours or 0.1 and 0.02 mmol/L FA for 48 h (P<0.05). CPT1 expression levels were significantly decreased after 48 h exposure，but SREBP-1c and COPⅠexpression levels were increased after both 24 h and 48 h exposures. Arf1 expression level was significantly increased after 24 h exposure. CONCLUSION：FA exposure could increase intracellular TG contents in HepG2 cells，and it may be associated with inhibition of fatty acid β oxidation and increased synthesis of triglyceride.

Key words: formaldehyde, triglyceride, CPT1, SREBP-1c, Arf1, COPⅠ

闫丹丹,白剑英,梁瑞峰,王幼萍,雷佩玉. 甲醛对HepG2细胞脂肪代谢的影响[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2013.06.003.

YAN Dan-dan，BAI Jian-ying，LIANG Rui-feng，WANG You-ping，LEI Pei-yu. Effects of formaldehyde on lipid metabolism in HepG2 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.06.003.

参考文献

[1] Tanaka K，Nishiyama K，Yaginuma H，et al. Formaldehyde exposure levels and exposure control measures during an anatomy dissecting course[J]. Kaibogaku Zasshi，2003，78 (2)：43-51.

[2] Tang X，Bai Y，Duong A，et al. Formaldehyde in China： production，consumption，exposure levels，and health effects[J]. Environ Int，2009，35 (8)：1210-1224.

[3] Cogliano VJ，Grosse Y，Baan RA，et al. Meeting report：summary of IARC monographs on formaldehyde，2-butoxyethanol，and 1-tert-butoxy-2-propanol[J]. Environ Health Perspect， 2005， 113 (9)：1205-1208.

[4] 张斌. 甲醛与食品安全[J]. 口岸卫生控制，2007，13 (2)： 47-48.

[5] 张文德. 食品中甲醛的来源及检测意义[J]. 中国食品卫生杂志，2006，18 (5)：455-459.

[6] Cikmaz S，Kutoglu T，Kanter M，et al. Effect of formaldehyde inhalation on rat livers：a light and electron microscopic study[J]. Toxicol Ind Health，2010，26 (2)：113-119.

[7] Schwartz DM，Wolins NE. A simple and rapid method to assay triacylglycerol in cells and tissues[J]. J Lipid Res， 2007，48 (11)：2514-2520.

[8] Collins JJ，John F，Esmen NA. An updated meta- analysis of formaldehyde exposure and upper respiratory tract cancers[J]. J Occup Environ Med，1997，39 (7)：639-651.

[9] Stone RA，Youk AO，Marsh GM，et al. Historical cohort study of US manmade vitreous fiber production workers：IV. Quantitative exposure-response analysis of the nested case- control study of respiratory systemcancer[J]. J Occup Environ Med，2001， 43： 779-792.

[10] Coggon D，Harris EC，Poole J，et al. Extended follow-up of a cohort of british chemical workers exposed to formaldehyde[J]. J Natl Cancer Inst，2003，95 (21)：1608-1615.

[11] Roush GC，Walrath J，Stayner LT，et al. Nasopharyngeal cancer， sinonasal cancer and occupation related to formaldehyde： a case control study[J]. J Natl Cancer Inst，1987，79 (6)： 1221-1224.

[12] 张玲，吕必华，江中发，等. 液态甲醛对HepG2细胞增殖影响及DNA损伤效应[J]. 环境与健康杂志，2010，27 (1)： 75-77.

[13] Marcsek ZL，Kocsis Z，Szende B，et al. Effect of formaldehyde and resveratrol on the viability of Vero，HepG2 and MCF-7 cells[J]. Cell Biol Int，2007，31 (10)：1214-1219.

[14] Shen L，Xiong Y，Wang DQ，et al. Ginsenoside Rb1 reduces fatty liver by activating AMP-activated protein kinase in obese rats[J]. J Lipid Res，2013，54 (5)：1430-1438.

[15] Morash AJ，McClelland GB. Regulation of carnitine palmitoy-ltransferase (CPT) I during fasting in rainbow trout (Oncorhynchus mykiss) promotes increased mitochondrial fatty acid oxidation[J]. Physiol Biochem Zool，2011，84 (6)：625-633.

[16] 秦艳，张晾，潘杰. 高脂饮食对非酒精性脂肪肝影响机制研究进展[J]. 实用肝脏病杂志，2010，13 (1)：72-74.

[17] Cohen JC，Horton JD，Hobbs HH. Human fatty liver disease： old questions and new insights[J]. Science，2011，332 (6037)： 1519-1523.

[18] Bonifacino JS，Benjamin SG. The mechanisms of vesicle budding and fusion [J]. Cell，2004，116 (2)：153-166.

[19] Lee MC，Miller EA，Goldberg J，et al. Bi-directional protein transport between the ER and Golgi[J]. Annu Rev Cell Dev Biol，2004，20：87-123.

[20] Gusarova V，Seo J，Sullivan ML，et al. Golgi-associated maturation of very low density lipoproteins involves conformational changes in apolipoprotein B，but is not dependent on apolipoprotein E[J]. J Biol Chem，2007，282 (27)： 19453-19462.

[21] Asp L，Magnusson B，Rutberg M，et al. Role of ADP ribosylation factor 1 in the assembly and secretion of ApoB-100-containing lipoproteins[J]. Arterioscler，Thromb，Vasc Biol，2005，25 (3)：566-570.

甲醛对HepG2细胞脂肪代谢的影响

Effects of formaldehyde on lipid metabolism in HepG2 cells

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