原癌基因Pim-3在胰腺癌组织中的表达及其与胰腺癌细胞增殖的相关性

doi:10.3969/j.issn.1004-616x.2014.01.010

癌变·畸变·突变

原癌基因Pim-3在胰腺癌组织中的表达及其与胰腺癌细胞增殖的相关性

陆平¹，李云鹏²，高岩³，刘景波¹，赵文杰¹，刘言¹，邢春艳¹

( 1. 齐齐哈尔医学院附属五院大庆龙南医院病理科，黑龙江大庆 163453 ；2. 齐齐哈尔医学院附属五院大庆龙南医院妇产科，黑龙江大庆 163453 ；3. 齐齐哈尔医学院附属五院大庆龙南医院营养科，黑龙江大庆 163453 )

收稿日期:2013-04-16 修回日期:2013-10-17 出版日期:2014-01-30 发布日期:2014-01-30
通讯作者: 陆平，E-mail：luping76@tom.com
作者简介:陆平 (1976- )，女，黑龙江省哈尔滨市人，硕士研究生，副主任医师，研究方向：临床肿瘤病理。Tel：13251661762；E-mail：luping76@tom.com

Expression of Pim-3 in pancreatic carcinoma and its correlation with cell proliferation

LU Ping¹，LI Yun-peng²，GAO Yan³，LIU Jing-bo¹，ZHAO Wen-jie¹，LIU Yan¹，XING Chun-yan¹

(1.Department of Pathology, Daqing Longnan Hospital of Heilongjiang Province and the Affiliated Hospital of Qiqihar Medical College, Daqing 163453; 2. Department of Gynaecology and Obstetrics, Daqing Longnan Hospital of Heilongjiang Province and the Affiliated Hospital of Qiqihar Medical College, Daqing 163453; 3. Department of Nutrition, Daqing Longnan Hospital of Heilongjiang Province and the Affiliated Hospital of Qiqihar Medical College, Daqing 163453, Heilongjiang, China)

Received:2013-04-16 Revised:2013-10-17 Online:2014-01-30 Published:2014-01-30
Contact: LU Ping，E-mail：luping76@tom.com

摘要/Abstract

摘要：

目的： 探讨胰腺癌组织中Pim-3的表达及其与癌细胞增殖的相关性。方法：采用免疫组化SP法检测15例正常胰腺组织和49例胰腺癌组织中Pim-3与增殖细胞核抗原 (PCNA)蛋白的表达，并计算PCNA增殖指数。结果：Pim-3在正常胰腺组织中不表达，而在胰腺癌组织中阳性表达，表达率为73.47％ (36/49)，明显高于正常胰腺组织 (P<0.01)。胰腺癌组织中PCNA的增殖指数 (32.54％±14.69％)明显高于正常胰腺组织 (6.25％±2.59％) (P<0.01)。且随胰腺癌细胞中Pim-3蛋白表达水平升高，肿瘤细胞增殖活性相应增加，Pim-3蛋白表达与PCNA增殖指数呈正相关 (r=0.726，P<0.01)。结论：Pim-3在胰腺癌组织中高表达，Pim-3与细胞的增殖密切相关，可能在胰腺癌的发生发展中起重要作用。

关键词: 胰腺癌, Pim-3, 增殖细胞核抗原

Abstract:

OBJECTIVE: To investigate the expression of Pim-3 in pancreatic carcinoma and its correlation with cell proliferation. METHODS：The streptavidin-peroxidase complex technique was used to examine the expression of Pim-3 and PCNA proteins in 15 normal pancreatic tissue and 49 pancreatic carcinoma and the proliferative index (PI) was calculated. RESULTS：In normal pancreatic tissue Pim-3 was not detected，whereas in pancreatic carcinoma the positive expression ratio of Pim-3 was 73.47% (36/49)．The positive rate of Pim-3 in pancreatic carcinoma was significantly higher than that in normal pancreatic tissue (P<0.01)．The PI in normal pancreatic tissue and pancreatic duct adenocarcinoma was 6.25%±2.59% and 32.54%±14.69%，respectively，also with a significant difference (P< 0.01)．With the increase in the expression of Pim-3 protein，the cell proliferating activity increased in the tumor cells. The Pim-3 expression level was positively correlated with PI (r=0.726，P<0.01). CONCLUSION：Pim-3 was overexpresed in human pancreatic carcinoma，and it may play important roles in the carcinogenesis and progression of pancreatic carcinoma.

Key words: pancreatic carcinoma, Pim-3, proliferating cell nuclear antigen

陆平，李云鹏，高岩，刘景波，赵文杰，刘言，邢春艳. 原癌基因Pim-3在胰腺癌组织中的表达及其与胰腺癌细胞增殖的相关性[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2014.01.010.

LU Ping，LI Yun-peng，GAO Yan，LIU Jing-bo，ZHAO Wen-jie，LIU Yan，XING Chun-yan. Expression of Pim-3 in pancreatic carcinoma and its correlation with cell proliferation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.01.010.

参考文献

[1] Mikkers H，Nawijn M，Allen J，et al. Mice deficient for all PIM kinases display reduced body size and impaired responses to hematopoietic growth factors[J]. Mol Cell Biol，2004，24 (13)：6104-6115.

[2] Deneen B，Welford SM，Ho T，et al. PIM3 proto-oncogene kinase is a common transcriptional target of divergent EWS/ETS oncoproteins[J]. Mol Cell Biol，2003，23 (11)：3897-3908.

[3] Amaravadi R，Thompson CB. The survival kinases Akt and Pim as potential pharmacological targets[J]. J Clin Invest，2005， 115 (10)：2618-2624.

[4] Stanger BZ，Stiles B，Lauwers GY，et al. Pten constrains centroacinar cell expansion and malignant transformation in the pancreas[J]. Cancer Cell，2005，8 (3)：185-195.

[5] She QB，Solit DB，Ye Q，et al. The BAD protein integrates survival signaling by EGFR/MAPK and PI3K/Akt kinase pathways in PTEN-deficient tumor cells[J]. Cancer Cell，2005，8 (4)： 287-297.

[6] Eichmann A，Yuan L，Bréant C，et al. Developmental expression of pim kinases suggests functions also outside of the hematopoietic system[J]. Oncogene，2000，19 (9)：1215-1224.

[7] Bullock AN，Debreczeni J，Amos AL，et al. Structure and substrate specificity of the Pim-1 kinase[J]. J Biol Chem， 2005，280 (50)：41675-41682.

[8] Aksoy I，Sakabedoyan C，Bourillot PY，et al. Self-renewal of murine embryonic stem cells is supported by the serine/threonine kinases Pim-1 and Pim-3[J]. Stem Cells，2007，25 (12)： 2996- 3004.

[9] Li YY，Popivanova BK，Nagai Y，et al. Pim-3，a proto-oncogene with serine/threonine kinase activity，is aberrantly expressed in human pancreatic cancer and phosphorylates bad to block bad-mediated apoptosis in human pancreatic cancer cell lines[J]. Cancer Res，2006，66 (13)：6741-6747.

[10] Fujii C，Nakamoto Y，Lu P，et al. Aberrant expression of serine/threonine kinase Pim-3 in hepatocellular carcinoma development and its role in the proliferation of human hepatoma cell lines[J]. Int J Cancer，2005，114 (2)：209-218.

[11] Liu LM，Deng H，Zhang JX，et al. Construction of a recombinant Pim3-expressing plasmid and expression and activity thereof：experiment with rats[J]. Zhonghua Yi Xue Za Zhi，2007，87 (36)：2567-2570.

[12] Yan B，Zemskova M，Holder S，et al. The PIM-2 kinase phosphorylates BAD on serine 112 and reverses BAD-induced cell death[J]. J Biol Chem，2003，278 (46)：45358-45367.

[13] Yang XY，Ren CP，Wang L，et al. Identification of differ-entially expressed genes in metastatic and non-metastatic nasopharyngeal carcinoma cells by suppression subtractive hybridization[J]. Cell Oncol，2005，27 (4)：215-223.

[14] Zheng HC，Tsuneyama K，Takahashi H，et al. Aberrant Pim-3 expression is involved in gastric adenoma-adenocarcinoma sequence and cancer progression[J]. J Cancer Res Clin Oncol， 2008，134 (4)：481-488.

原癌基因Pim-3在胰腺癌组织中的表达及其与胰腺癌细胞增殖的相关性

Expression of Pim-3 in pancreatic carcinoma and its correlation with cell proliferation

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