四名健康志愿者外周血总RNA辐射敏感分子的筛选

doi:10.3969/j.issn.1004-616x.2026.02.005

摘要/Abstract

摘要： 目的： 筛选不同剂量⁶⁰Co γ射线照射后人外周血中差异表达的关键基因，为辐射损伤筛选新的生物标志物。方法： 采用⁶⁰Co γ射线对4名健康成人志愿者外周血分别进行不同剂量（0、2、4、6和8 Gy）的照射。在照射后收集各组全血，提取总RNA，并进行RNA高通量测序（RNA-seq），随后利用生物信息学方法对测序数据进行分析。使用R语言软件筛选各组间的差异表达基因，并获得共同差异基因集。进一步通过Metascape平台对共同差异基因进行GO功能富集分析及KEGG通路富集分析。利用STRING数据库构建蛋白相互作用网络，并通过Cytoscape软件筛选关键基因。结果： 测序数据分析结果显示，γ射线照射后人外周血共筛选出281个共同差异基因，包括89个lncRNAs和192个mRNAs。共同差异基因GO富集主要包括淋巴细胞活化、免疫效应过程的正向调控、炎症反应、细胞组分大小的调控等；KEGG通路富集在细胞因子-细胞因子受体相互作用、病毒致癌通路、Th17细胞分化、核糖体通路、T细胞受体信号通路、血小板活化通路和细胞间黏着连接通路；蛋白相互作用网络筛选得到10个关键基因CD80、CD40、IL2RA、CCR7、NFKB1、TP53、IL21R、STAT6、PDCD1、SLAMF1。结论： 本次筛选的10个关键基因是潜在的辐射损伤生物标志物。

关键词: 电离辐射, RNA测序, 辐射损伤, 生物标志物

Abstract: OBJECTIVE: To investigate changes in differentially expressed genes in peripheral blood after in vitro exposure to different doses of⁶⁰Co γ rays and to identify new biomarkers for radiation damage.METHODS: Peripheral blood from four healthy adult volunteers were irradiated with different doses of⁶⁰Co γrays（0, 2, 4, 6, and 8 Gy）. Total RNA samples were extracted from irradiated blood using RNA high-throughput sequencing（RNA seq）. The sequencing data was analyzed using bioinformatics methods. The R language software was used to screen differentially expressed genes between groups, and a common set of differentially expressed genes was identified. In addition, GO functional enrichment analysis and KEGG pathway enrichment analysis were performed on the differentially expressed genes using the Metascape platform.A protein interaction network was constructed using STRING database and key genes were screened using the Cytoscape software. RESULTS: A total of 281 common and differentially expressed genes were screened,including 89 lncRNAs and 192 mRNAs. The enrichment of these expressed genes was shown by GO to mainly included lymphocyte activation, positive regulation of immune effector process, positive regulation of immune response, inflammatory response,regulation of cellular component size. The KEGG pathway was enriched in cytokine cytokine receptor interactions, viral carcinogenesis, Th17 cell differentiation, ribosomes, T cell receptor signaling pathways,platelet activation,and intercellular adhesion junctions. Protein-protein interaction network screening identified 10 key genes: CD80, CD40, IL2 RA, CCR7, NFKB1, TP53, IL21R, STAT6,PDCD1,and SLAMF1. CONCLUSION: The 10 key genes might be useful biomarkers for demonstration of radiation damage.

Key words: ionizing radiation, RNA sequencing, radiation damage, biomarkers

中图分类号:

TL71

张成思, 姜红, 龙高群, 李远航, 田祝娟. 四名健康志愿者外周血总RNA辐射敏感分子的筛选[J]. 癌变·畸变·突变, 2026, 38(2): 113-118.

ZHANG Chengsi, JIANG Hong, LONG Gaoqun, LI Yuanhang, TIAN Zhujuan. Screening for radiation-responsive molecules in blood RNA from four healthy volunteers[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2026, 38(2): 113-118.

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