Abstract: To clone a genomic DNA f ragment containing mouse glucocorticoid receptor ( GR) gene for the const ruction of an inducible gene targeting vector used in the establishment of a GR gene dificient mouse model. The mouse genomic library was screened by using a 562bp PCR product from GR gene exon Ⅱas a probe. Six positive clones were got after screening. The DNA of positive clones was profoundly characterized by sequencing , rest riction mapping and Southern blotting to get the right f ragment . A 6. 5 kb DNA f ragment was separated from the C10 clone. The gene f ragment contains entire GR gene exon Ⅱ, and two flanking f ragment s which are 3. 9 kb and 1. 4 kb respectively can be used as homologous arm in the const ruction of the targeting vector.

Key words: gene targeting, glucocorticoid receptor gene