应用胞质分裂阻滞微核法检测三卤甲烷和卤乙腈的遗传毒性

doi:10.3969/j.issn.1004-616x.2012.01.010

›› 2012, Vol. 24 ›› Issue (1): 42-45.doi: 10.3969/j.issn.1004-616x.2012.01.010

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Genotoxicity detection of trihalomethanes and haloaceto-nitriles using cytokinesis-block micronucleus test

LIAO Jing，LIU Ai-lin，CAO Wen-cheng，LU Wen-qing

Department of Occupational and Environmental Health, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei, China

Received:2011-06-01 Revised:2011-09-16 Online:2012-01-30 Published:2012-01-30
Contact: LU Wen-qing

Abstract

Abstract: OBJECTIVE: To investigate the genotoxicity induced by two types of disinfection by-products (DBPs) trihalomethanes (chloroform，bromodichloromethane，chlorodibromomethane and bromoform) and haloacetonitriles (trichloroacetonitrile and dibromoacetonitrile). METHODS：Human hepatocellular carcinoma cell line HepG2 cells were treated with the DBPs mentioned above at four concentrations to evaluate their effects on the formation of micronuclei and nuclear division index (NDI) by means of cytokinesis-block micronucleus test (CBMNT). Dimethyl sulfoxide and benzo(a)pyrene were used as solvent control and positive control，respectively. RESULTS：Chloroform， bromodichloromethane and chlorodibromomethane were found to induce statistically significant increases in the frequency of micronuclei at the concentrations of 10 000 and 3 000 μmol/L(P＜0.05 )，while bromoform did not show similar effect on HepG2 cells in comparison with the solvent control. Trichloroacetonitrile and dibromoacetonitrile also induced statistically significant increases in the frequency of micronuclei and decreases in NDI at the lowest observed adverse effect levels of 2 000 and 30 μmol/L(P＜0.05 )，respectively. CONCLUSION：CBMNT could be used to detect the genotoxicity of trihalomethanes and haloacetonitriles，which were found to cause chromosome breakage in HepG2 cells.

Key words: trihalomethanes, acetonitriles, micronuclei, disinfection

LIAO Jing，LIU Ai-lin，CAO Wen-cheng，LU Wen-qing. Genotoxicity detection of trihalomethanes and haloaceto-nitriles using cytokinesis-block micronucleus test[J]. , 2012, 24(1): 42-45.

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Genotoxicity detection of trihalomethanes and haloaceto-nitriles using cytokinesis-block micronucleus test

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