Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (4): 295-297.doi: 10.3969/j.issn.1004-616x.2010.04.011

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The single and double skeletal staining of rat and rabbit fetuses by batch-wise procedure

JING Shu-fang; WU Chun-qi;LIAO Ming-yang   

  1. National Beijing Center for Drug Safety Evaluation and Research，Beijing Institute of Pharmacology and Toxicology，Beijing 100850，China
  • Received:2010-03-03 Revised:2010-04-27 Online:2010-07-30 Published:2010-07-30
  • Contact: LIAO Ming-yang

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Abstract: OBJECTIVE: To develop a new method for quick, effective and batch-wise skeletal staining of the fetuses of rats and rabbits. METHODS: After setting up of relevant staining equipments and improvement of staining procedures, the fetuses underwent single and double skeletal staining by the new batch-wise procedures, and the staining quality of fetal skeletons was compared with those stained with routine procedures used in our lab. RESULTS: With the new method for skeletal staining, the workload for the staff and consumables used could be reduced, with comparative staining effects as those for the routine procedures. CONCLUSION: This batch-wise skeletal staining procedure could be used in drug safety evaluation.

Key words: fetal skeleton, batch-wise staining, single staining, double staining, rat, rabbit

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