天元甘露液对小鼠肠道5种微生物菌群定植的调节作用

doi:10.3969/j.issn.1004-616x.2025.02.010

Abstract

Abstract: OBJECTIVE：To establish a mouse model of intestinal microbiota dysbiosis and to observe whether Tianyuan manna and/or yoghurt would regulate colonization of 5 kinds of intestinal microflora.METHODS：The SPF Kunming mice were divided into five groups：blank control group，model (yoghurt) control group，Tianyuan manna (containing yoghurt) 4.2，8.3 and 16.6 g/kg groups，with 10 mice in each group. The model was established by oral administration of 200 mg/kg norfloxacin for 7 consecutive days. After the model was established，Tianyuan manna and/or yoghurt were given by gavage for 16 consecutive days. The number of intestinal bifidobacteria and Lactobacillus (2 beneficial bacteria)，Clostridium perfringens，Enterococcus and Enterobacter (3 harmful bacteria) were counted by pouring dish method，and compared with the blank control group and the model control group. RESULTS：After the norfloxacin treatment，the number of viable intestinal microflora was significantly reduced compared with the blank control group (P<0.01)，indicating that the model was successful. After administration of Tianyuan manna and/or yoghurt，two beneficial bacteria exceeded the number of intestinal viable bacteria in normal mice (P<0.01)，and three harmful bacteria were lower (P<0.05 or P<0.01). Compared with the model control group，the number of harmful bacteria in each dose group of Tianyuan manna was significantly reduced (P<0.05 or P<0.01)，and the number of beneficial bacteria (Bifidobacterium) in the 8.3 g/kg group was slightly reduced (P<0.05). CONCLUSION：In the process balance and imbalance of intestinal flora imbalance，Tianyuan manna demonstrated regulatory effect on the colonization of five kinds of microbial flora.

Key words: Tianyuan manna, mice, gut microbiota, microbiota colonization

CLC Number:

R965

YANG Yu, MA Yue, LI Jingyun, SHAN Chun. Regulatory effects of Tianyuan manna on colonization of five intestinal microbial flora in mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2025, 37(2): 153-156.

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Regulatory effects of Tianyuan manna on colonization of five intestinal microbial flora in mice

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