Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (3): 194-196.doi: 10.3969/j.issn.1004-616x.2009.03.009

Previous Articles     Next Articles

The Relationship between the Expression of DNA Methyltransferase and Multidrug

ZHOU Jian-meng1,2, YUAN Jian-hui2, JI Na-na2, LIU Jian-jun2, ZHUANG Zhi-xiong1,2   

  1. 1.School of Public Health,Sun Yat-Sen University,Guangzhou 510080; 2. Shenzhen Center for Disease Control and Prevention,Shenzhen 518020,China
  • Received:2008-10-20 Revised:2009-01-20 Online:2009-05-30 Published:2009-05-30

PDF (PC)

2199

Abstract: BACKGROUND AND AIM: We investigated the relationship between the expression of DNA methyltransferase(DNMT) and multidrug resistance gene ABCG2 in breast cancer, in order to further study the epigenetic mechanism of ABCG2 expression. MATERIALS AND METHODS: Use real-time transcription-PCR(RT-PCR) to quantify DNMTs and ABCG2 mRNA expressions in 22 breast cancer and their matching adjacent tissues. We also used Spearman rank test to analyze the relationship between mRNA levels of the DNMTs and target gene ABCG2 involved in the DNMT pathway. RESULTS:Compared with adjacent tissues, the mRNA expressions of DNMTs and ABCG2 were markedly higher in breast cancer,especially DNMT3B,which was significantly higher than that of DNMT1 and DNMT3A. There was a negative relationship between DNMT3B and ABCG2(r=-0.664,P<0.01) in breast cancer. CONCLUSION:DNMT3B may play an important role in the epigenetic mechanism of ABCG2 expression in breast cancer,providing new scientific basis for searching the therapeutic target to reverse the multidrug resistance caused by ABCG2.

Key words: breast cancer, DNMT, ABCG2, real-time quantitative RT-PCR