Carcinogenesis, Teratogenesis & Mutagenesis ›› 1998, Vol. 10 ›› Issue (1): 11-015.doi: 10.3969/j.issn.1004-616x.1998.01.005

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STUDY ON THE GENOTOXICITY OF ETHYLENE OXIDE Ⅷ STUDY OF DNA QUANTITATIVE ANALYSIS IN THE MOUSE HEPATOCYTES

Wang J inmao1 ,Zhang Shuren2 ,Lu J ingfen2 ,Zhong Kun2 ,Zhang Ling2 ②   

  1. 1 Zhejiang Medical University , 2 S hanghai medical College f or Heal th S taf f , S hanghai 200237
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-01-30 Published:1998-01-30

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Abstract: With the digital image analytic system ,this study is to quantitate the DNA in hepatocytes nuclei of exposed mice with different doses of ethylene oxide. The result s showed that the quantity of DNA in the hepatocytes nuclei of exposed groups is higher than that in the cont rol roup. It is worth noticing ,the DNA quantity in male groups is obviously higher than that in the females.By comparing the male exposed groups with cont rol group and the male exposed groups with female exposed groups ,their DNA quantity in the nuclei of hepatocytes are both significant different . The K- S test P < 0. 01. The data suggested that EtO not only caused the duplication of DNA in the experimental mice hepatocytes but also induced the tendency of approching malignant changes in the hepatocytes. The hepatocytes in the male mice are more sensitive to EtO than those in the female.

Key words: ethylene oxide, hepatocyte, quantitative analysis of DNA