HPV18 E6 E7 基因诱发的人胎儿食管上皮永生化和恶性转化细胞端粒长度和端粒酶活性

doi:10.3969/j.issn.1004-616x.2001.03.001

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2001, Vol. 13 ›› Issue (3): 137-140.doi: 10.3969/j.issn.1004-616x.2001.03.001

TELOMERE LENGTH AND TELOMERASE ACTIVITY IN IMMORTALIZED AND MALIGNANTLY TRANSFORMED HUMAN EMBRYONIC ESOPHAGEAL EPITHELIAL CELL LINES BY E6 AND E7 GENES OF HPV 18 TYPE

XU Li-yan, SHEN Zhong-ying, LI En-min, etal .

Pathological Department, Shantou University Medical College, Shantou 515031, China

Received:2000-09-03 Revised:2001-04-06 Online:2001-07-30 Published:2001-07-30

Abstract

Abstract: Purpose : To study the relationship of the telomere length and telomerase activity to immortalization or malignant t ransformation of the immortalized esophageal epithelial cell line (SHEE) and malignantly t ransformed cell line (SHEEC) induced by E6 and E7 genes of HPV 18 type. Methods : Using SHEE and SHEEC cell lines , the telomere length (mean length of telomere rest riction f ragment s , TRF) was examined by Southern blotting , the telomerase activity by the PCR2based telomeric repeat amplification protocol ( TRAP) and the expression of human telomerase reverse t ranscriptase ( hTERT) and human telomerase RNA ( hTR) by reverse t ranscription polymerase chain reaction(RT - PCR) . Results : The telomere length in the SHEE and SHEEC was significantly shorter than that in the normal esophageal epithelial tissue , but maintained a stable length. The telomerase activity and it s subunit hTERT and hTR were observed in the SHEE and SHEEC. Conclusion : A possible cause of stable division of SHEE and SHEEC is telomerase reactivitied , which maintains the telomere at a shortened and stable length.

Key words: telomere length, telomerase, esophageal cancer cell line, malignant t ransformation, immotalization

CLC Number:

XU Li-yan, SHEN Zhong-ying, LI En-min, etal .. TELOMERE LENGTH AND TELOMERASE ACTIVITY IN IMMORTALIZED AND MALIGNANTLY TRANSFORMED HUMAN EMBRYONIC ESOPHAGEAL EPITHELIAL CELL LINES BY E6 AND E7 GENES OF HPV 18 TYPE[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2001, 13(3): 137-140.

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TELOMERE LENGTH AND TELOMERASE ACTIVITY IN IMMORTALIZED AND MALIGNANTLY TRANSFORMED HUMAN EMBRYONIC ESOPHAGEAL EPITHELIAL CELL LINES BY E6 AND E7 GENES OF HPV 18 TYPE

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