甲基丙烯酸环氧丙酯致人支气管上皮恶性转化细胞DNA修复基因点突变的研究

doi:10.3969/j.issn.1004-616x.2009.01.001

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (1): 1-005.doi: 10.3969/j.issn.1004-616x.2009.01.001

Point Mutation of DNA Repair Genes of Human Bronchial Epithelial Cells Malignant Transformation Induced by Glycidyl Methacrylate

DONG Lin, LI Ying, WANG Quan-kai, SUN Jin-xiu, XU Jian-ning

National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention,Beijing 100050,China

Received:2008-08-29 Revised:2008-11-16 Online:2009-01-30 Published:2009-01-30

Abstract

Abstract: BACKGROUND AND AIM: To examine the mutation of DNA repair genes of human bronchial epithelial cells malignant transformation induced by glycidyl methacrylate. MATERIALS AND METHODS: To evaluate the mutation of DNA repair genes XRCC1, hMSH2, XPD and XRCC3 by polymerase chain reaction-restriction fragments length polymorphism(PCR-RFLP), and the result was verified by the DNA sequencing. RESULTS: The mutation of hMSH2 IVS12-6 (T>C) was observed, while mutations in the other genes were not found.The results from PCR-RFLP and DNA sequencing were consistent. CONCLUSION:The mutation of DNA repair genes hMSH2 IVS12-6 (T>C)might be important and an initation step during the malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate.

Key words: glycidyl methacrylate, DNA repair genes, point mutation, mglignant transformation

DONG Lin, LI Ying, WANG Quan-kai, SUN Jin-xiu, XU Jian-ning. Point Mutation of DNA Repair Genes of Human Bronchial Epithelial Cells Malignant Transformation Induced by Glycidyl Methacrylate[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2009, 21(1): 1-005.

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Point Mutation of DNA Repair Genes of Human Bronchial Epithelial Cells Malignant Transformation Induced by Glycidyl Methacrylate

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