维生素E琥珀酸酯对人胃癌细胞增殖分化和热休克蛋白70表达的影响

doi:10.3969/j.issn.1004-616x.2005.02.006

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2005, Vol. 17 ›› Issue (2): 87-89.doi: 10.3969/j.issn.1004-616x.2005.02.006

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Effect of Vitamin E Succinate on Cell Proliferation， Differentiation and Expression of Heat Shock Protein 70 in Human Gastric Carcinoma

ZHAO Lan;LI Hong-wei;WU Kun

Department of Nutrition and Food Hygiene,Harbin Medical University, Harbin Heilongjiang 150001， China

Received:2004-03-11 Revised:2004-06-28 Online:2005-03-30 Published:2005-03-30
Contact: ZHAO Lan

Abstract

Abstract: BACKGROUND＆AIM: To study the effect of vitamin E succinat(VES) on the human gastric carcinoma SGC-7901 cell and the possible mechanism. MATERIAL AND METHODS: Using cell culture in vitro, the effect of VES at the dose of 5,10 μg/ml were measured by MTT method for cell growth rate, by flow cytometry for cell cycle distribution, by spectrophotometer for enzyme activity, by western blotting for the expression of HSP70(heat shock protein 70). RESULTS: After treated with VES, SGC-7901 cells were led to growth inhibition, cell cycle arrest, AKP, LDH activity suppressant. Western blotting analysis demonstrated that the expression of HSP70 in SGC-7901 cells was markedly decreased. CONCLUSION: HSP70 is involved in VES induced growth inhibition and differentiation of SGC-7901 cells, and associate with cell cycle arrest.

Key words: vitamin E succinate, gastric carcinoma, cell cycle, heat shock protein 70

ZHAO Lan, LI Hong-wei, WU Kun. Effect of Vitamin E Succinate on Cell Proliferation， Differentiation and Expression of Heat Shock Protein 70 in Human Gastric Carcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2005, 17(2): 87-89.

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Effect of Vitamin E Succinate on Cell Proliferation， Differentiation and Expression of Heat Shock Protein 70 in Human Gastric Carcinoma

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