六价铬对大鼠肝细胞体外的毒作用

doi:10.3969/j.issn.1004-616x.2005.06.006

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2005, Vol. 17 ›› Issue (6): 343-345.doi: 10.3969/j.issn.1004-616x.2005.06.006

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Cytotoxicity of Hexavalent Chromium to Rat Hepatocytes in Vitro

WANG Xiao-feng; FU Wen-yu; ZHU Xin; XU Li-hong

Department of Biochemistry and Molecular Biology, School of Medicine

Received:2004-12-14 Revised:2005-01-24 Online:2005-11-30 Published:2005-11-30
Contact: XU Li-hong

Abstract

Abstract: BACKGROUND ＆ AIM: To study the toxicity of hexavalent chromium on rat hepatocytes . MATERIAL AND METHODS: Rat hepatocytes BRL－3A were treated with hexavalent chromium at concentrations of 0, 0.1, 1, 10 and 100 μmol/L Viability was assessed by 3_[4,5_dimethylthiazol_2_yl]_2,5 diphenyl tetrazolium bromide(MTT) method and flow cytometry was used to analyze cell apoptosis. RESULTS: Viability decreased with the increasing concentration of hexavalent chromium. Cell apoptosis induced by hexavalent chromium also showed in a dose_dependent pattern. CONCLUSION: These data indicated that hexavalent chromium had a damaging effect on hepatocytes and induced apoptosis at the concentrations used in the present study.

Key words: hexavalent chromium, hepatocytes, toxicity

WANG Xiao-feng, FU Wen-yu, ZHU Xin, XU Li-hong. Cytotoxicity of Hexavalent Chromium to Rat Hepatocytes in Vitro[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2005, 17(6): 343-345.

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Cytotoxicity of Hexavalent Chromium to Rat Hepatocytes in Vitro

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