两种人类肝细胞系评价饮水氯化消毒副产物MX致 DNA损伤作用的敏感性

doi:10.3969/j.issn.1004-616x.2007.01.010

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2007, Vol. 19 ›› Issue (1): 33-035.doi: 10.3969/j.issn.1004-616x.2007.01.010

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Susceptibility of Two Human-Derived Hepatic Cell Lines in Evaluating DNA Damage Induced by MX

WU Jian-jun1, LIU Ai-lin2, ZOU Ya-ling2, ZHOU Li-hong2, LU Wen-qing2

1.Institute for Chemical Carcinogenesis of Guangzhou Medical College, Guangzhou 510182; 2. Department of Occupational and Environmental Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei, China

Received:2005-10-17 Revised:2006-02-28 Online:2007-01-30 Published:2007-01-30

Abstract

Abstract: BACKGROUND ＆ AIM: To study on the susceptibility of two human-derived hepatic cell lines in evaluating DNA damage induced by 3-chloro-4-dichloromethyl-5-hydroxy-2(5H)-furanone(MX). MATERIALS AND METHODS: Using single cell gel electrophoresis assay, to evaluate the DNA damage induced by MX in human hepatocellular carcinoma cell lines(HepG2) and human fetal hepatocytes (L-02). RESULTS: MX could induce HepG2 and L-02 DNA single strand breaks at the concentrations of 30 and 100 μmol/L, respectively. The length of DNA migration induced by MX increased significantly in comparison to solvent control (DMSO) in a dose-response manner(P<0.05). Moderate or greater DNA damages induced by MX in HepG2 were more serious than that of L-02(χ2=20.985；8.0；11.97， P<0.05). CONCLUSION: HepG2 was more susceptible than L-02 cells to DNA damage induced by MX evaluated by using single cell gel electrophoresis assay.

Key words: HepG2, L-02, single cell gel electrophoresis assay, DNA damage

CLC Number:

R394.6，R994.6

WU Jian-jun, LIU Ai-lin, ZOU Ya-ling, ZHOU Li-hong, LU Wen-qing. Susceptibility of Two Human-Derived Hepatic Cell Lines in Evaluating DNA Damage Induced by MX[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2007, 19(1): 33-035.

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Susceptibility of Two Human-Derived Hepatic Cell Lines in Evaluating DNA Damage Induced by MX

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