Carcinogenesis, Teratogenesis & Mutagenesis ›› 2007, Vol. 19 ›› Issue (2): 146-148.doi: 10.3969/j.issn.1004-616x.2007.02.019

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Utilization of Wright-Giemsa Mixed Stain in Cr(Ⅵ) –Induced HepG2 Cells Micronuclei Test

BIAN Huan-feng1， AN Fei-yun1，ZHONG Cai-gao1,， LIAO Chun-hua2   

  1. 1.Department of Health Toxicology, School of Public Health, Central South University， Changsha 410078, China; 2. Jiangxi Center for Disease Prevention and Control, Nanchang 330029, China
  • Received:2006-06-13 Revised:2006-10-30 Online:2007-03-30 Published:2007-03-30
  • Contact: ZHONG Cai-gao

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Abstract: BACKGROUND ＆ AIM: To explore the influence of different concentrations and different proportions of Wright-Giemsa dye on its staining effect, through application to micronucleus test of HepG2 cells induced by hexavalent chromium[Cr(Ⅵ)]. MATERIALS AND METHODS: HepG2 cells were stained with different proportions of Wright-Giemsa dye and for different stain time, and their influence on staining effects were assessed. RESULTS: Better results could be obtained when cells were stained with a concentration of Wright-Giemsa at 3∶1 for 3-5 minutes. CONCLUSION: Wright-Giemsa stain could be applied to micronucleus test with favorable effects.

Key words: Wright stain, Giemsa stain, Wright-Giemsa stain, binuclei, HepG2 cells

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