微囊藻毒素LR对人FL和HL7702细胞PP2A各亚基转录水平的影响

doi:10.3969/j.issn.1004-616x.2009.06.004

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (6): 422-425.doi: 10.3969/j.issn.1004-616x.2009.06.004

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Effect of Microcystin LR on the Transcription of Subunits of PP2A of FL and HL7702

LIANG Jing;HUANG Pu;FU Wen-yu;XU Li-hong

Department of Biochemistry and Genetics, Medical School, Zhejiang University, Hangzhou 310058,Zhejiang, China

Received:2009-04-28 Revised:2009-06-22 Online:2009-11-30 Published:2009-11-30
Contact: XU Li-hong

Abstract

Abstract: BACKGROUND AND AIM: To investigate the effects of microcystin LR on the mRNA expression of protein phosphotase 2A subunits of human amniotic (FL) cells and human hepatocyte HL7702 cells. MATERIALS AND METHODS: After treated with 100 nmol/L MCLR for 24 h, the mRNA expression of protein phosphotase 2A subunits of human amniotic cells and human hepatocyte cells was measured by real time PCR. RESULTS: The changes of subunits expression were diverse in human amniotic cells but increased significantly in human hepatocyte cells. CONCLUSION: The special effects of MCLR on HL7702 cells could be account for its hepatotoxicity.

Key words: protein phosphotase 2A, microcystin LR, mRNA, real time PCR

CLC Number:

X503.1

LIANG Jing, HUANG Pu, FU Wen-yu, XU Li-hong. Effect of Microcystin LR on the Transcription of Subunits of PP2A of FL and HL7702[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2009, 21(6): 422-425.

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Effect of Microcystin LR on the Transcription of Subunits of PP2A of FL and HL7702

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