白血病Flt3基因近膜区突变的检测和分析

doi:10.3969/j.issn.1004-616x.2011.04.012

›› 2011, Vol. 23 ›› Issue (4): 294-297.doi: 10.3969/j.issn.1004-616x.2011.04.012

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Analysis of mutation in the juxtamembrane domain of Flt3 gene in leukemia

ZHAO Min，WANG Xiao-hua，LI Tie-chen，ZHAO Yue-hua*

1. Department of Hematology, the Second Hospital of Wuhu, Affiliated to Wannan Medical College, Wuhu 241001; 2. Department of Genetics, Wannan Medical College, Wuhu 241001, Anhui, China

Received:2010-12-13 Revised:2011-03-31 Online:2011-07-30 Published:2011-07-30
Contact: ZHAO Min

Abstract

Abstract: OBJECTIVE: To identify the relationship between the pathogenesis of leukemia and Flt3 gene by detecting the mutations in juxtamembrane domain coded by Flt3. METHODS：We tested exons14，15 of Flt3 gene using PCR，PCR-SSCP and gene sequence in 60 leukeamia patients’ bone marrow samples. RESULTS：8 Flt3-ITD mutations，another 3 Flt3-L576P point mutations were found in juxtamembrane domain in 60 patients，including 1 patient with a deletion of intron 14. CONCLUSION：Flt3-L576P is a new point mutation site，which might be associated with the pathogenesis and development of leukemia.

Key words: Flt3, leukemia, juxtamembrane domain, mutation

ZHAO Min，WANG Xiao-hua，LI Tie-chen，ZHAO Yue-hua*. Analysis of mutation in the juxtamembrane domain of Flt3 gene in leukemia[J]. , 2011, 23(4): 294-297.

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Analysis of mutation in the juxtamembrane domain of Flt3 gene in leukemia

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