多溴联苯醚BDE-3、BDE-47和BDE-209的体外遗传毒性评价

doi:10.3969/j.issn.1004-616x.2020.01.003

Abstract

Abstract: OBJECTIVE: To evaluate in vitro genotoxicity of three representative Polybrominated Diphenyl Ethers (PBDEs):BDE-3,BDE-47 and BDE-209. METHODS: In vitro alkaline comet assay,in vitro cytokinesis-block micronucleus (CBMN) test and TK gene mutation assay were conducted using TK6 cells. These assays detect DNA damage,chromosome aberrations and gene mutation. Five doses of each PBDEs were used:60,90,120,180 and 240 μmol/L for BDE-3;60,120,180,200 and 240 μmol/L for BDE-47;and 24,40,120,180 and 240 μmol/L for BDE-209;while DMSO was used as negative control. RESULTS: Comparing with the negative control groups,exposure to the three PBDEs did not cause DNA damage,i.e. an increase in comets tail length,tail DNA intensity and tail moment (P > 0.05);and also did not increase the frequency of micronucleus (P > 0.05). However,the same exposures increased TK gene mutation frequencies significantly (P < 0.05) and in a dose-dependent manner ([R_BDE-3²]=0.85,[R_BDE-47²]=0.85,[R_BDE-209²]=0.90). The mutagenicity of BDE-47 was strongest. CONCLUSION: BDE-3,BDE-47 and BDE-209 were mutagenic to TK6 cell.

Key words: polybrominated diphenyl ethers, comet assay, micronucleus test, TK gene mutation assay, genotoxicity

CLC Number:

R994.6

CAO Yiyi, LIU Weiying, YOU Xinyue, XI Jing, Chen Ruixue, ZHANG Xinyu, LUAN Yang. In vitro genotoxicity evaluation of BDE-3, BDE-47 and BDE-209[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 15-20,28.

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In vitro genotoxicity evaluation of BDE-3, BDE-47 and BDE-209

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