乙嘧酚磺酸酯诱发CHO和L5178Y细胞基因突变的研究

doi:10.3969/j.issn.1004-616x.2012.02.014

›› 2012, Vol. 24 ›› Issue (2): 141-144.doi: 10.3969/j.issn.1004-616x.2012.02.014

• 检测研究 • Previous Articles Next Articles

HPRT gene mutation in ovarian cells and TK gene mutation in lymphoma cells induced by Bupirimate

ZHANG Kang，ZHUANG Wan，CHENG Gui-hong，LI Long

1. Department of Health Toxicology, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei; 2. Inspection and Quarantine Technology Center, Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361026, Fujian, China

Received:2011-05-23 Revised:2011-11-27 Online:2012-03-30 Published:2012-03-30
Contact: LI Long

Abstract

Abstract: OBJECTIVE: To investigate the effects of bupirimate on HPRT gene of ovarian cells and the TK gene of lymphoma cells of mouse in vitro culture system. METHODS：CHO cells and L5178Y cells were treated with bupirimate at different concentrations (5，15，50，150 μg/ml). Determination of cytotoxicity，cell inoculation efficiency，relative survival rate and mutation frequency were performed. RESULTS：The relative survival rate and suspension growth rate of CHO cells and L5178Y cells decreased significantly with the increasing doses of bupirimate. But the mutation frequencies of HPRT and TK genes induced by bupirimate (5-150 μg/ml) were not higher than that of spontaneous mutation frequency of L5178Y cells. CONCLUSION：Bupirimate did not show obvious mutagenic effects on HPRT gene in CHO cells and TK gene in L5178Y cells.

Key words: HPRT gene, TK gene, mutation, bupirimate

ZHANG Kang，ZHUANG Wan，CHENG Gui-hong，LI Long. HPRT gene mutation in ovarian cells and TK gene mutation in lymphoma cells induced by Bupirimate[J]. , 2012, 24(2): 141-144.

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HPRT gene mutation in ovarian cells and TK gene mutation in lymphoma cells induced by Bupirimate

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