2，4-D丁酯对金鱼血清过氧化物酶和酯酶同工酶的影响

doi:10.3969/j.issn.1004-616x.2014.04.005

Abstract

Abstract:

OBJECTIVE: To explore the effect of herbicide 2，4-D butyl ester on the expressions of peroxidase (POD) and esterase (EST) in Carassius auratus serum. METHODS：0.5，1.0，1.5，2.0 mL/L concentrations of 2，4-D butyl ester (according to its 24 h LC50) and 0.9% saline control group were prepared. Carassius auratus received intraperitoneal injection of 0.1 mL/g dose of each concentration. Tail blood of Carassius auratus and the supernatant of centrifugation were collected after 24 h. SDS-polyacrylamide gel electrophoresis was used to test the toxicity on POD and EST. The experimental data were analysed with ANOVA. RESULTS：POD and EST enzyme activities were inhibited at low concentrations(0.5 mL/L)，induced at medium concentrations(1.0 mL/L) and decreased at high concentrations (1.5，2.0 mL/L). Compared with the control group. the effect of 2，4-d butyl ester on migration rate of EST isoenzyme was greater than POD. CONCLUSION：2，4-D butyl ester affected the activities of POD and EST in Carassius auratus and 2，4-d butyl ester changed the EST isoenzyme mobility.

Key words: 2, 4-D butyl ester, Carassius auratus, serum, POD, EST

ZHOU Gui-qin，ZHANG Xiao-hong，HUO Hai-yan，WANG Mei-mei，GUO Li-min，WEN Hai-ying. Effect of 2, 4-D butyl ester on POD and EST isoenzymes expression in Carassius auratus serum[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.04.005.

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Effect of 2, 4-D butyl ester on POD and EST isoenzymes expression in Carassius auratus serum

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