Orai1对鼻咽癌细胞侵袭和上皮-间充质转化的影响及其机制

doi:10.3969/j.issn.1004-616x.2021.06.003

Abstract

Abstract: OBJECTIVE: The study was aimed to investigate mechanisms of Orai1 on nasopharyngeal carcinoma cell invasion and epithelial-mesenchymal transformation (EMT) in vitro. METHODS: A nasopharyngeal epithelial cell line (NP69) and nasopharyngeal carcinoma cell lines (CNE1,CNE2,HONE1 and 5-8F) were cultured. Real-time quantitative PCR and Western blot were used to detect expressions of Orai1 mRNA and protein. The short hairpin RNA (shRNA) plasmid targeting Orai1 was used to down-regulate expressions of Orai1 in CNE2 cells. Store-operated Ca²⁺ entry (SOCE) was detected by calcium imaging,cell migration and invasion were detected by Transwell chamber,and E cadherin,N cadherin and Vimentin were detected by real-time quantitative PCR and Western blot. RESULTS: Orai1 was expressed in NP69,CNE1,CNE2,HONE1 and 5-8F cells,and the expression of Orai1 was higher in CNE2 cells than that in NP69 cells (P<0.05). The mRNA and protein levels of ORAI1 were significantly decreased in CNE2 cells transfected with Orai1 shRNA (P<0.05). SOCE,migration/invasion and EMT were significantly inhibited in Orai1 shRNA transfected CNE2 cells (P<0.05). CONCLUSION: Blockage of ORAI1 expression inhibited SOCE,migration/invasion and EMT process of nasopharyngeal carcinoma cells. Therefore, Orai1 might be a new target and a new direction for the treatment of nasopharyngeal carcinoma metastasis.

Key words: Orai1, Ca²⁺, nasopharyngeal carcinoma, metastasis, epithelial-mesenchymal transformation

CLC Number:

R73-37

GUO Zhaomeng, ZHANG Hua, ZHANG Peng. Mechanisms of Orai1 on invasion and epithelial-mesenchymal transformation in nasopharyngeal carcinoma cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 420-425.

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Mechanisms of Orai1 on invasion and epithelial-mesenchymal transformation in nasopharyngeal carcinoma cells

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