NDRG2基因在贲门腺癌中的甲基化状态及表达

doi:10.3969/j.issn.1004-616x.2014.04.008

Abstract

Abstract:

OBJECTIVE: To investigate the promoter methylation status of N-myc downstream-regulated gene 2 (NDRG2) and its correlation with mRNA and protein expressions of the gene in gastric cardia adenocarcinoma (GCA). METHODS：MSP method was used to measured the promoter methylation status of NDRG2 in 97 GCA tumor tissues and corresponding normal tissues. RT-PCR and immunohistochemistry were used to examine mRNA and protein expression，respectively，of NDRG2 in 97 GCA tumor tissues and corresponding normal tissues. RESULTS：The promoter methylation frequency of NDRG2 in GCA tumor tissues (49.5%) was significantly increased in comparison to that in corresponding normal tissues (5.1%) (P<0.05). The methylation frequency of NDRG2 in stages Ⅲ and Ⅳ tumor tissues was significantly higher than that in stages Ⅰ and Ⅱ tumor tissues. The methylation frequency of NDRG2 in poorly differentiated group was significantly higher than that in well and moderately differentiated group (P<0.05). NDRG2 mRNA expression in tumor tissues was significantly lower than that in corresponding normal tissues (P<0.05). Positive protein expression of NDRG2 in tumor tissues (44.3%，43/97) was significantly lower than that in corresponding normal tissues (94.8%，92/97) (P<0.01). The protein expression of NDRG2 was inversely correlated with its promoter methylation status in GCA tissues. CONCLUSION：Promoter hypermethylation of NDRG2 may be one of the mechanisms that lead to gene inactivation in gastric cardia adenocarcinoma.

Key words: cardia, adenocarcinoma, N-myc downstream-regulated gene 2, methylation, expression

MA Hong-liang,HAN Li-jie，GUO Wei，GUO Yan-li，SHEN Su-peng，KUANG Gang. Promoter methylation status and expression of NDRG2 gene in gastric cardia adenocarcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.04.008.

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Promoter methylation status and expression of NDRG2 gene in gastric cardia adenocarcinoma

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