饮水途径镉暴露后小鼠体内镉分布研究

doi:10.3969/j.issn.1004-616x.2024.06.008

Abstract

Abstract: OBJECTIVE： This study aimed to elucidate the distribution，accumulation and toxicity of cadmium in the blood，esophagus，liver，and kidneys of mice following subchronic exposure of cadmium in drinking water. METHODS： For this study，27 pathogen-free (SPF)-grade C57BL/6J mice were randomly assigned to a control，low-dose (10 mg/kg CdCl₂)，and high-dose groups (75 mg/kg CdCl₂). The control group received standard feed and double-distilled water，while the exposed groups were given standard feed and a solution containing the corresponding concentration of CdCl₂. The low-dose group was exposed to cadmium for 8，12，16，and 20 weeks，and the high-dose group for 16 weeks，with three mice per time point. Whole blood was collected from the orbital cavity of mice of the control group at 8 and 12 weeks，the low-dose group at 8，16，and 20 weeks，and the high-dose group at 16 weeks. Mice were sacrificed according to the experimental time points，and esophageal，liver，and kidney tissues were collected. Cadmium levels were measured using inductively coupled plasma mass spectrometry (ICP-MS). RESULTS： Blood cadmium levels in the treated groups were significantly higher than in the control group (P<0.01). The blood cadmium levels in the low-dose group were significantly increased compared to the control (P<0.01)，but there was no statistically significant difference among the different exposure time points within the group (P>0.05). At the 16-week time point，the blood cadmium levels in the high-dose group were significantly higher than in the low-dose group (P<0.01). The esophageal cadmium concentration in the low-dose group was higher than in the control group at all exposure time points (P<0.05 or P<0.01)，with the highest concentration at 20 weeks (67.7 μg/L)，which was statistically significant compared to 8 weeks (44.0 μg/L) and 12 weeks (35.2 μg/L) (P<0.05 or P<0.01). The esophageal cadmium concentration in the high-dose group (129.2 μg/L) at 16 weeks was significantly higher than in the low-dose group (47.9 μg/L，P<0.01). In the low-dose group，cadmium concentrations in liver and kidney tissues increased from 12-16 weeks and 8-16 weeks，respectively，compared to the control group (P<0.05 or P<0.01)，reaching a peak at 16 weeks (P<0.01) before declining. At 16 weeks，there were statistically significant differences in the cadmium concentrations of liver and kidney tissues among all dose groups，with the highest concentrations in the high-dose group (P<0.01). Cadmium levels were quantifiable in the blood，esophagus，liver，and kidneys of both control and cadmium-treated groups，with the highest to lowest order of cadmium content being kidney>liver>esophagus>blood. CONCLUSION： Dietary cadmium exposure resulted in the accumulation of cadmium in the blood，esophagus，liver，and kidney of mice，exhibiting a definite time- and dose-dependent effect.

Key words: cadmium, mice, accumulation, inductively coupled plasma mass spectrometry, esophagus

CLC Number:

R994.6

HE Lihua, LUO Jianan, ZHANG Xiarong, CHEN Jiongyu, XIE Bingmeng, PENG Lin. Distribution of cadmium in mice after exposure to cadmium via drinking water[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2024, 36(6): 470-475,496.

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Distribution of cadmium in mice after exposure to cadmium via drinking water

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