Carcinogenesis, Teratogenesis & Mutagenesis ›› 2006, Vol. 18 ›› Issue (1): 6-008.doi: 10.3969/j.issn.1004-616x.2006.01.002

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Experimental Study on Inhibition of pEgr-TNFα Gene Therapy Combined with Radiotherapy on Esophageal Cancer Cells

WU Cong-mei1,HUANG Tian-hua1,WU De-sheng1,XIE Qing-dong1,LI De-rui2,CHEN Xiu-ru2,XU Xiao-hu3   

  1. 1.Research Center of Reproductive Medicine, Shantou University Medical College, Shantou 515041,Guangdong, China; 2. Tumor Hospital, Shantou University Medical College, Shantou 515041,Guangdong,China; 3. Forensic Department, Shantou University Medical College, Shantou 515041,Guangdong,China
  • Received:2004-09-15 Revised:2004-11-05 Online:2006-01-30 Published:2006-01-30
  • Contact: HUANG Tian-hua

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Abstract: BACKGROUND ＆ AIM: To study the expression of pEgr-TNFα induced by ionizing irradiation in stably transfected esophageal cancer cells and to study the inhibition effects of pEgr-TNFα combined with ionizing irradiation on esophageal cancer cells. MATERIAL AND METHODS: Esophageal cancer cell line EC9706 was transfected with pEgr-TNFα plasmids and selected through G418 to get stable expression cells. TNFα expression was detected by ELISA. The inhibition effect of pEgr-TNFα was observed after different doses of X-ray irradiation. RESULTS: pEgr-TNFα plasmids were transfected into EC9706 cells with stable expression. High dose and low dose X-ray irradiation could all enhance the expression of TNFα, about 6－6.3 times of control group (P<0.001). Cells stably transfected was given 0.075 Gy and 2 Gy X-ray irradiation, number of cells was less than non-transfected group and sham group (P<0.01～0.001) 8 d later. CONCLUSION: pEgr-TNFα gene-radiotherapy can significantly inhibit the growth of esophageal cancer cells in vitro.

Key words: pEgr-TNFα plasmid;X-ray irradiation, esophageal cancer cell;low dose irradiation

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