Carcinogenesis, Teratogenesis & Mutagenesis ›› 1997, Vol. 09 ›› Issue (1): 23-28.doi: 10.3969/j.issn.1004-616x.1997.01.008

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ESTABL ISHM ENT OF A M ETHOD FOR ID ENT IFY ING PCD AND ITS USE IN STUDY ING THE EFFECTS OF GNRH- A ON PCD IN RAT TEST IS GERM CELLS

Zhu Honghong, Huang Xingshu   

  1. Toxicology Department of Zhejiang Medical University ,Hangzhou 310031
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-01-30 Published:1997-01-30

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Abstract: In order to evaluate the reproductive toxicity of environmental horm ones and explore them echanism of male infertility,the present experiment used in situ ending labeling (ISEL) assay to analyse the effects of environmental chemicals on apoptosis Firstly established the method of ISEL ,and secondly used the ISEL to measure the reproduction tox icity of GnRH-A.ISEL has the advantages of high specifity and sensitivity, and good repetition. In situ analysis of developing test is demonstrated that sperm atocytes and sperm atogon ia constituted the major PCD cell types That PCD rate in GnRHA group was apparently higher than that of the control group showed that GnRH -A is an apogen exerting reproduction toxicity.

Key words: Programmed cell death (PCD), In situ end labeling (ISEL) Testis germ cell, Gonadotroping releasing hormone analogue(GnRH -A )