辐射致急性小鼠衰老模型的建立

doi:10.3969/j.issn.1004-616x.2007.06.017

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2007, Vol. 19 ›› Issue (6): 491-493.doi: 10.3969/j.issn.1004-616x.2007.06.017

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Establishment of an Acute Aging Model in Mice Induced by γ-ray Irradiation

YANG Xue-kang， HAI Chun-xu， LIANG Xin， ZHAO Hai-long， BAI Hua

Department of Toxicology，the Fourth Military Medical University，Xi'an 710033，China

Received:2006-11-22 Revised:2007-03-20 Online:2007-11-30 Published:2007-11-30
Contact: HAI Chun-xu

Abstract

Abstract: BACKGROUND ＆ AIM： To establish a convenient acute aging model by γ-ray irradiation． MATERIALS AND METHODS： 45 mice were divided into 3 groups：the control group，the model 1 group irradiated by 3 Gy×8 times and the model 2 group irradiated by 6 Gy×4 times. Complete blood count, thymus weight index，spleen weight index， activity of SOD，the contents of MDA and lipofuscin were determined.The expression of β-galactosidase was evaluated by staining at the end of 50 days experiment． RESULTS： Compared with control group, the complete blood count，thymus weight index and spleen weight index all decreased(P<0.05),the activity of SOD also decreased(P<0.05),but the contents of MDA and lipofuscin were increased(P<0.05).The β-galactosidase activity of hepatic cells was enhanced in the aging mice model．CONCLUSION：The acute aging model by γ-ray irradiation was successfully established, and it was a feasible method.

Key words: aging, free radical, model

CLC Number:

R332

YANG Xue-kang, HAI Chun-xu, LIANG Xin, ZHAO Hai-long, BAI Hua. Establishment of an Acute Aging Model in Mice Induced by γ-ray Irradiation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2007, 19(6): 491-493.

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Establishment of an Acute Aging Model in Mice Induced by γ-ray Irradiation

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