Carcinogenesis, Teratogenesis & Mutagenesis ›› 1998, Vol. 10 ›› Issue (6): 331-335.doi: 10.3969/j.issn.1004-616x.1998.06.001

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EFFECTS OF VARIOUS ANTIOXIDANTS ON UV2INDUCED THE FORMATION OF HUMAN SKIN'S CYCLOBUTYL PRIMIDINE DIMERS

E Xiaofei1 , Zhu Xudong2 , Li J ing1 , Liu Yang1 , Sun Xinyan3 , Wang Bingxian1   

  1. 1Department of Envi ronmental Heal th , School of Public Heal th , China Medical University 　2 Department of Center L aboratory , The f i rst Clinical College , 3Department of A naesthesia , S henyang 　110001
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-11-30 Published:1998-11-30

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Abstract: To define further the role of reactive oxygen species ( ROS) in ult raviolet radiation (UV)2induced the formation of Human skin’s CPD , experiment s were performed using alkaline gelelect rophoresis technique. Purified DNA was incubated with antioxidant s. After DNA was exposed to UV , T4 Endonuclease V was added , elect rophoresis was taken , CPD was measured by Gel Imaging and Image Analysis. It was found that antioxidant s exhibited some inhibitory effects on the formation of CPD ,β2carotene and superoxide dismutase ( SOD) are more obvious than GSH and CAT , but none of which were able to block the formation completely. The result s suggest that the formation of CPD is only partly dependent on ROS.

Key words: ROS, Antioxidant, CPD, Ult raviolet radiation