Carcinogenesis, Teratogenesis & Mutagenesis ›› 2008, Vol. 20 ›› Issue (2): 139-142.doi: 10.3969/j.issn.1004-616x.2008.02.016

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Experimental Research on Anti-semen Antibody of Golden Hamster as Spermicide

CHEN De-yu1,2 ，LIU Li-min1，XIE Qing-dong1，HUANG Tian-hua1   

  1. 1. Research Center for Reproductive Medicine, Department of Cell Biology and Genetics, Shantou University Medical College, Shantou 515041, Guangdong; 2. Department of Biology, Fuyang Normal College, Fuyang 236032,Anhui, China
  • Received:2007-11-26 Revised:2008-01-12 Online:2008-03-30 Published:2008-03-30
  • Contact: HUANG Tian-hua

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Abstract: BACKGROUND AND AIM: To study the feasibility of anti-semen antibody as spermicide. MATERIALS AND METHODS: Anti-semen antibody of golden hamster was prepared to incorporate with golden hamster semen,then sperm motility rate analysis, spermagglutination test, sperm mitochondrion function analysis, in vitro fertilization, intrauterine sperm injection and post mate endovaginal sperm motility analysis were performed to assess the inhibitory effect of anti-semen antibody on sperm in vitro and in vivo. RESULTS: Anti-semen antibody could agglutinate and immobilize sperm completely. In experiment group and control group, the average of sperm mitochondrion fluorescence intensity was 180.28±82.24 and 309.74±148.37, the rate of in vitro fertilization was 0.9％ and 37.50％, the rate of fertilization in intrauterine sperm injection test was 1％ and 15.0％, the sperm motility rate in post mate endovaginal sperm motility analysis was 0 and 60.0％, respectively.All the differences between the experiment group and the control group were statisti cal significant (P<0.01). CONCLUSION: Anti-semen antibody could agglutinate and immobilize sperm in vitro, in uterine and in vagina, and it could be used as a potential spermicide.

Key words: anti-semen antibody, spermicide, spermagglutination, in vitro fertilization, golden hamster