Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (3): 206-209.doi: 10.3969/j.issn.1004-616x.2009.03.012

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Pilot Study on the Mechanism of CNE Cell Apoptosis Induced by Indole-3-carbinol

ZHU Wei1, WEI Qing2, YANG Guang-yu1, YANG Xing-fen3,   

  1. 1. Department of Toxicology, Guangzhou Center for Disease Control and Prevention, Guangzhou 510080；2.Department of Preventive Medicine, School of Public Health, Zhongshan University, Guangzhou 510080;3. Guangdong Center for Disease Control and Prevention, Guangzhou 510300,China
  • Received:2008-11-14 Revised:2009-01-06 Online:2009-05-30 Published:2009-05-30

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Abstract: BACKGROUND AND AIM: To study the effect of indole-3-carbinol(I3C) in CNE cells as well as the underlying mechanisms. MATERIALS AND METHODS: The inhibition of cell proliferation was assayed by WST-1 agent, and the apoptosis was shown with Hochest 33258 and TUNEL staining. To study the mechanism of apoptosis, the expression of the cleaved caspase-9 and the cleaved caspase-3 were detected with western immublotting assay. RESULTS: I3C could inhibit the proliferation of CNE cells and apoptosis could be induced by I3C in a dose-dependent manner. Furthermore, the expressions of cleaved caspase-9 and cleaved caspase-3 appeared to be increased significantly as compared with those of the control. CONCLUSION: I3C could induce apoptosis of CNE cells dose-dependently, and caspase-9 and caspase-3 pathways might be involved.

Key words: indole-3-carbinol, CNE cell, caspase-9, caspase-3