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doi:10.3969/j.issn.1004-616x.2010.02.005

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (2): 100-103.doi: 10.3969/j.issn.1004-616x.2010.02.005

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Influence of hepatitis B virus surface protein on sperm motility and mitochondrial membrane potential

ZHOU Xiao-ling;SUN Ping-nan;KANG Xiang-jin;LIU Dong-ling;XIE Qing-dong

Department of Biology, Shantou University Medical College, Shantou 515041, Guangdong, China

Received:2009-11-18 Revised:2009-12-30 Online:2010-03-30 Published:2010-03-30
Contact: ZHOU Xiao-ling

Abstract

Abstract: OBJECTIVE: Sperm of HBV patients displayed lower motility and higher ratio of apoptosis and necrosis when compared with those of normal persons. The mechanisms involved in the process, however, remain obscure. This article was aimed at investigating the influence of hepatitis B virus surface protein (HBs) on sperm mitochondrial membrane potential (MMP). METHODS: Sperm motility was analyzed after incubation with HBs in vitro. Sperm MMP was assayed by using JC-1 dyeing and flow cytometry. RESULTS: The percentage of sperm class a＋b significantly decreased after incubation with 100 μg/ml HBs in vitro. CONCLUSION: HBV might adversely impact on sperm motility and MMP by HBs protein.

Key words: hepatitis B virus surface protein, human sperm, mitochondrial membrane potential

CLC Number:

ZHOU Xiao-ling, SUN Ping-nan, KANG Xiang-jin, LIU Dong-ling, XIE Qing-dong. Influence of hepatitis B virus surface protein on sperm motility and mitochondrial membrane potential[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2010, 22(2): 100-103.

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Influence of hepatitis B virus surface protein on sperm motility and mitochondrial membrane potential

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