Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (2): 149-152.doi: 10.3969/j.issn.1004-616x.2009.02.017

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Preparation of the Monoclonal Antibody Specific to Mig-2Protein by Genetic Immunization

HU Yuan-qing1,TU Chang-chun2,WEI Guang-sen3, ZHANG Hong-quan4,   

  1. 1. Anhui Science and Technology University, Anhui Key Laboratory of Poultry Epidemic Disease Prevention and Surveillance,Fengyang 233100,Anhui,China;2.Military Veterinary Research Institute, Academy of Military Medicine, Changchun 130062, Jilin,China;3. Lanzhou Veterinary Research Institute ,Chinese academy of Agricultural Sciences, Lanzhou 730046,Gansu,China;4.Karolinska Institutet, Department of Microbiology, Pathology, and Immunology, SE-141 86 Huddinge,Sweden
  • Received:2008-06-30 Revised:2008-09-16 Online:2009-03-30 Published:2009-03-30

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Abstract: BACKGROUND AND AIM: Mig-2 protein is an important moleculer in the process of tumor formation, and is a target for tumor therapy. To search new effective ways to diagnose and treat malignant tumors, we prepared the McAbs of Mig-2 protein. MATERIALS AND METHODS: BALB/c mice were immunized with recombinant plasmid of Mig-2(the vector is P3XFLAG-CMV-10).By cell fusion and cell cloning, the McAbs of Mig-2 protein were prepared. RESULTS: Three strains of hybridoma cells 3C4,4H2 and 1F8 all secreting a subclass of IgM, anti-mig-2 antibody,were obtained after fusion followed by three or four screenings. The a scitic fluid McAbs were prepared by injecting the hybridoma cells into mice abdomen. The indirect ELISA results showed that ascitic McAbs revealed high affinity with the Mig-2 protein at the titer of 1∶2.4×104-1∶4.8×104,and the titer of cell supernatant was 1∶32-1∶512. CONCLUSION: Prepared McAbs of Mig-2 were specific and stable agent for tumor therapy research.

Key words: genetic immunization, monoclonal antibody, Mig-2, tumor therapy