Carcinogenesis, Teratogenesis & Mutagenesis ›› 2008, Vol. 20 ›› Issue (4): 295-298.doi: 10.3969/j.issn.1004-616x.2008.04.012

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Protective Effects of Huangqi on Vascular Endothelial Cells Exposed to High Gluose

FAN Ke1,CHEN Pei_hong2,WU Qing3   

  1. 1. Liyuan Hospital,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430000;2. Wuhan Hospital of Tradional Chinese Medicine, Wuhan 430014; 3.Institute of Hematotogy Union Hospital, Tongji Medical Colllege of Huazhong University of Science and Technology, Wuhan 430022,China
  • Received:2007-09-24 Revised:2008-03-09 Online:2008-07-30 Published:2008-07-30

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Abstract: BACKGROUND AND AIM: To evaluate the protective effects of Huangqi on the damage of high glucose on vascular endothelial cell(VEC_304)in vitro． MATERIALS AND METHODS: The model of human umbilical vein endothelial cells (VEC_304) damage was induced by 50％ glucose for 4 h.The cells were pretreated with three concentrations of Huangqi solution for 24 h,before they were exposed to glucose. MTT and protein levels in cells were determined after 4 h. The expression of VEGF was investigated by RT_PCR and immunohistochemistry methods. RESULTS: MTT and protein levels in cells of the normal control group amd Huangqi_treated groups were higher than in glucose_damaged group without huangqi(P<0.05). The expression of VEGF was inhibited by glucose. CONCLUSION: Huangqi could protect the damages of VEC_304 endothelial cells induced by high glucose.

Key words: Huangqi, glucose, vascular endothelial cells 304